FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3288
Total Bases476.6 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC60

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG141042.88321167883212No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAA99530.261557177615572No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCCTGCATAGACCAA1003.0413625304136254No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTCTGCATAGACCAAGTC571.7335766423357664No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC541.6423357664233578No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG200.6082725060827251No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG190.5778588807785888No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG170.5170316301703163No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG140.4257907542579075No Hit
CCATTGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG130.3953771289537713No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATGATGCCTGCATAGACCAAG110.33454987834549876No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCCTGCATAGACCAAG90.2737226277372263No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCCTGCATAGACCAAG90.2737226277372263No Hit
CCATAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG90.2737226277372263No Hit
AGATCAGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG90.2737226277372263No Hit
AGATCATGTACGTCGGGGACGTCCGCAGTGTCACACAGAAGCATATCCAG90.2737226277372263No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG90.2737226277372263No Hit
CCATTAGTAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG80.24330900243309003No Hit
AGATATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG80.24330900243309003No Hit
AGATCATGTACGTCGGGGACGTCCGCGGCGTCACACAGAAGCATATCCAG80.24330900243309003No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG80.24330900243309003No Hit
CCATTAGTGAGTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG80.24330900243309003No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATCCTGCATAGACCAAG80.24330900243309003No Hit
CCTTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG70.21289537712895376No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCGCACAGAAGCATATCCAG70.21289537712895376No Hit
CCATTAGTGAGCTGGCCAACCCTTGATGATTGATGCCTGCATAGACCAAG70.21289537712895376No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGAGCCTGCATAGACCAAG70.21289537712895376No Hit
CCATTAGTGAGCTGGCCAACCCTGGATGATTGATGCCTGCATAGACCAAG70.21289537712895376No Hit
CCATTAGTGAGCTGGCCACCCTGTGATGATTGATGCCTGCATAGACCAAG70.21289537712895376No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCCTGCATAGACCAA60.18248175182481752No Hit
CCATTAGTGACTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG60.18248175182481752No Hit
CCATTAGTGAGCTGGCCAACCCTGTGAGATTGATGCCTGCATAGACCAAG60.18248175182481752No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACGGAAGCATATCCAG60.18248175182481752No Hit
AGATCTGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG60.18248175182481752No Hit
AGATCATGACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG60.18248175182481752No Hit
AGATCATGTCGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG50.15206812652068127No Hit
CCATTAGTGAGCTGGCAAACCCTGTGATGATTGATGCCTGCATAGACCAA50.15206812652068127No Hit
CCATTAGTGAGCTGGCCAACCTGTGATGATTGATGCCTGCATAGACCAAG50.15206812652068127No Hit
AGACATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG50.15206812652068127No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTATGCCTGCATAGACCAAG50.15206812652068127No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATGTCCAG50.15206812652068127No Hit
AGATCATTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG50.15206812652068127No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTCTGCATAGACCAAGTCT50.15206812652068127No Hit
CCATTAGTGAGCTGGCCAACCCTGTGTGATTGATGCCTGCATAGACCAAG50.15206812652068127No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAGGCATATCCAG40.12165450121654502No Hit
CCATTATGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG40.12165450121654502No Hit
CCATTAGTAGCTGGCCAAACCTGTGATGATTGATGCCTGCATAGACCAAG40.12165450121654502No Hit
CCATTAGGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG40.12165450121654502No Hit
GATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG40.12165450121654502No Hit
CCATTAGTGAGCTGGCCAACCCGTGATGATTGATGCCTGCATAGACCAAG40.12165450121654502No Hit
CCATTAGTGAGCTGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG40.12165450121654502No Hit
AGATCATGTACGTCGGGGTCGTCCGCAGCGTCACACAGAAGCATATCCAG40.12165450121654502No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG40.12165450121654502No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCAAAGACCAA40.12165450121654502No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGCCACACAGAAGCATATCCAG40.12165450121654502No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGTATATCCAG40.12165450121654502No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGGTGCCTGCATAGACCAA40.12165450121654502No Hit
AATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG40.12165450121654502No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)