FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1383
Total Bases200.5 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA85061.46059291395517No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGAA805.784526391901663No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCA715.1337671728127265No Hit
GCATCAATCATCACAGGGTTGGCCAGCCACTAATGGGAATCCTGTAGAAC100.7230657989877078No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGAATCCTGTAGAAC100.7230657989877078No Hit
GCATCAATCATCACAGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC90.6507592190889371No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTATGGGAATCCTGTAGAAC90.6507592190889371No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC90.6507592190889371No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC80.5784526391901663No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGAATCCTGTAGAAC80.5784526391901663No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT70.5061460592913956No Hit
GCATCAATCATCACAGGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGA70.5061460592913956No Hit
GCATCAATCATCACAGGGTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.43383947939262474No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAAGGGAATCCTGTAGAAC60.43383947939262474No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.43383947939262474No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACAATGGGAATCCTGTAGAAC60.43383947939262474No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGAATCCTGTAGAACCAT50.3615328994938539No Hit
GCATCAATCATCACAGGGTTGGCCAGTCACTAATGGGAATCCTGTAGAAC50.3615328994938539No Hit
GCATCAATCTCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.3615328994938539No Hit
GCATCAATCATCACAGGGTTGGCAGCTCACTAATGGGAATCCTGTAGAAC40.28922631959508316No Hit
ACGTTGCCTTTATCCTCCCACAGAGCCTTGGCATGCTCATAGAATTCGGG40.28922631959508316No Hit
GCATCAATCATCACAGGGTTGGCCAGCTACTAATGGGAATCCTGTAGAAC40.28922631959508316No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.28922631959508316No Hit
GCATCAATCATCACAGGGTTGGCCACTCACTAATGGGAATCCTGTAGAAC30.21691973969631237No Hit
ACGTTGCCTTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACC30.21691973969631237No Hit
ACGTTGCCTTTATCCTCGACGTACATGATCTGAATCCTGTAGAACCATGT30.21691973969631237No Hit
GCTCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.21691973969631237No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGAATCCTGTAGAAC30.21691973969631237No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC30.21691973969631237Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGGGTTGGCCAGCTCCTAATGGGAATCCTGTAGAAC30.21691973969631237No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.21691973969631237No Hit
GATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.21691973969631237No Hit
GCATCAATCATCACAGGTTTGGCCACTCACTAATGGGAATCCTGTAGAAC20.14461315979754158No Hit
GCATCAAACATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA20.14461315979754158No Hit
GCATCAATCATCAAGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.14461315979754158No Hit
CATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.14461315979754158No Hit
GCATCATCATCACAGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.14461315979754158No Hit
ACGTTGCCTTTATCCCCCCGACGTACATGATCTGAATCCTGTAGAACCAT20.14461315979754158No Hit
ACGTTGCCTTTATCCCCGACGTACATGATCTGAATCCTGTAGAACCATGT20.14461315979754158No Hit
GCATCAATCATCACGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.14461315979754158No Hit
CGTTGCCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCAT20.14461315979754158No Hit
GCATCAATCATCACAGGGTTAGCCAGCTCACTAATGGGAATCCTGTAGAA20.14461315979754158No Hit
GCATCAATAATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA20.14461315979754158No Hit
ACGTTGCCTTTATCCTCCCCCACGTACATGATCTGAATCCTGTAGAACCA20.14461315979754158No Hit
ACGTTGCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCAT20.14461315979754158No Hit
GCACAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.14461315979754158No Hit
GCATCAATCATCACAGGGTTGACCAGCTCACTAATGGGAATCCTGTAGAA20.14461315979754158No Hit
GCATCAATCATCACAGGGTTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGG20.14461315979754158No Hit
ACGTTGCCTTTATCGTCCCCGACGTACATGATCTGAATCCTGTAGAACCA20.14461315979754158No Hit
GCATCAATCACACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.14461315979754158No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCATAATGGGAATCCTGTAGAAC20.14461315979754158No Hit
GCATCAATCATAACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA20.14461315979754158No Hit
GCATCAATCATCACAGGTTTGGCCAGCTACTAATGGGAATCCTGTAGAAC20.14461315979754158No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)