FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970
Total Bases284.2 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT78239.69543147208122No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA56328.578680203045685No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGAA492.487309644670051No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCA301.5228426395939088No Hit
ACGTTGCCTTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGG201.015228426395939No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT120.6091370558375634No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGAATCCTGTAGAAC100.5076142131979695No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC70.3553299492385787No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGAATCCTGTAGAAC70.3553299492385787No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGGAATCCTGTAGAA70.3553299492385787No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGCTCTGGT70.3553299492385787No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG70.3553299492385787No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT60.3045685279187817No Hit
GCATAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.25380710659898476No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT50.25380710659898476No Hit
GCATCAATCATCACAGGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGA50.25380710659898476No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG50.25380710659898476No Hit
GCATCAATCATCACAGGGTTGGCCAGCTACTAATGGGAATCCTGTAGAAC50.25380710659898476No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCAGGT50.25380710659898476No Hit
CATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.20304568527918782No Hit
GCATCAATCATCACAGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.20304568527918782No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.20304568527918782No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG40.20304568527918782No Hit
GCATCAATCATCACAGGGTTGGCCAGCTAACTAATGGGAATCCTGTAGAA40.20304568527918782No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCGGGT40.20304568527918782No Hit
ACGATGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT40.20304568527918782No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCATAATGGGAATCCTGTAGAAC40.20304568527918782No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.20304568527918782No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGTGCCCATTCGATCTGGT30.15228426395939085No Hit
ACTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG30.15228426395939085No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCGTTCGATCTGGT30.15228426395939085No Hit
GCATCAATCATCACAGGGTTGGCAGCTCACTAATGGGAATCCTGTAGAAC30.15228426395939085No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG30.15228426395939085No Hit
GCTCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.15228426395939085No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGA30.15228426395939085No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGCCGCCTGTAGAA30.15228426395939085No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTAGATCTGGT30.15228426395939085No Hit
ACGTTGACTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT30.15228426395939085No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGACCCATTCGATCTGGT30.15228426395939085No Hit
ACGTTGCCTATATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT30.15228426395939085No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACAATGGGAATCCTGTAGAAC30.15228426395939085No Hit
GATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.15228426395939085No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGTT20.10152284263959391No Hit
ACATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA20.10152284263959391No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA20.10152284263959391Clontech SMARTer II A Oligonucleotide (100% over 24bp)
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCTCATTCGATCTGGT20.10152284263959391No Hit
GCATCAATCATCACAGGGTTGGCAAGCTCACTAATGGGAATCCTGTAGAA20.10152284263959391No Hit
GCATCAATCATCACAGGGTTGGCCAGCCACTAATGGGAATCCTGTAGAAC20.10152284263959391No Hit
ACGTTGCCTTTATCCTCCCAGATCCATGAGTGGGGCCCATTCGATCTGGT20.10152284263959391No Hit
ACGTTGCCTTTATCCTCCCAGATCTAGGAGTGGGGCCCATTCGATCTGGT20.10152284263959391No Hit
ACGTTGCCTTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACC20.10152284263959391No Hit
GGATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA20.10152284263959391No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGCCCATTCGATCTGGTG20.10152284263959391No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG20.10152284263959391No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT20.10152284263959391No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGC20.10152284263959391No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGCGGGGCCCATTCGATCTGGT20.10152284263959391No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGG20.10152284263959391No Hit
ACGTTGCCTTTATCCTCCCAGACCCAGGAGTGGGGCCCATTCGATCTGGT20.10152284263959391No Hit
GCATCAATCATCACAGGGTTGGCCAGATCACTAATGGGAATCCTGTAGAA20.10152284263959391No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGAGGCCCATTCGATCTGGT20.10152284263959391No Hit
GCATCAATCACCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA20.10152284263959391No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAAAGGGAATCCTGTAGAA20.10152284263959391No Hit
ACGTTGCCTTTATCCTCCCGATCCAGGAGTGGGGCCCATTCGATCTGGTG20.10152284263959391No Hit
GCATCAATCTCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.10152284263959391No Hit
ACGTTGCCTTTATACTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT20.10152284263959391No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAT20.10152284263959391No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.10152284263959391No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAGTGGGAATCCTGTAGAA20.10152284263959391No Hit
ACGTTGCCTTTATCCACCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT20.10152284263959391No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC20.10152284263959391Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGACCTGGT20.10152284263959391No Hit
GCATCAATCATCACAGGGTTGACCAGCTCACTAATGGGAATCCTGTAGAA20.10152284263959391No Hit
ACGTAGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT20.10152284263959391No Hit
CCGTTGCCTTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGG20.10152284263959391No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATATGGT20.10152284263959391No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGACCATTCGATCAGGT20.10152284263959391No Hit
GCATCAATCATCATGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.10152284263959391No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCTATTCGATCTGGT20.10152284263959391No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCCTAATGGGAATCCTGTAGAAC20.10152284263959391No Hit
GCATCAATCACACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.10152284263959391No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAGCTGGT20.10152284263959391No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)