FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000
Total Bases144.9 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA65265.2No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGAA727.199999999999999No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCA202.0No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGGAATCCTGTAGAA60.6No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAAGGGAATCCTGTAGAAC60.6No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.6No Hit
GCATCAATCATCACAGGGTTGGCCACTCACTAATGGGAATCCTGTAGAAC60.6No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.6No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGAATCCTGTAGAAC60.6No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGAATCCTGTAGAAC50.5No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACAATGGGAATCCTGTAGAAC50.5No Hit
GCATCAATCATCACCGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA40.4No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA40.4Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.4No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCCTAATGGGAATCCTGTAGAAC40.4No Hit
GCTCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.4No Hit
GCATCAATCATCACGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.3No Hit
GCATAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.3No Hit
GCATCAATCATCACAGGGTTGGCCAGTCACTAATGGGAATCCTGTAGAAC30.3No Hit
GCATCAATCACACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.3No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.3No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGAATCCTGTAGAACCAT30.3No Hit
GCATCAATCATCATAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA20.2No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA20.2Clontech Universal Primer Mix Long (96% over 26bp)
GCATCAATCTCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.2No Hit
GCATCAATCATCACAGGGTTGGCCAGCCACTAATGGGAATCCTGTAGAAC20.2No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC20.2Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATAAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA20.2No Hit
GCATCAATCATCACAGGGTTGGGCAGCTCACTAATGGGAATCCTGTTGAA20.2No Hit
GCATCAACATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.2No Hit
GCATCAATCATCACAGGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGA20.2No Hit
GCATCAATCATCACAGGGTTGACCAGCTCACTAATGGGAATCCTGTAGAA20.2No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGAATCCTGTAGAACC20.2No Hit
GCATCAATCATCACAGGGTTGGCAGCTCACTAATGGGAATCCTGTAGAAC20.2No Hit
GCATCAATCATCACAGGGTTGGACAGCTCACTAATGGGAATCCTGTAGAA20.2No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTATGGGAATCCTGTAGAAC20.2No Hit
GCATCAATCATCACAGGGTTGGCCAGCTACTAATGGGAATCCTGTAGAAC20.2No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGAAGAA20.2No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCATAATGGGAATCCTGTAGAAC20.2No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGAATCCTGTAGAAC20.2No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)