FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267
Total Bases183.7 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA77461.089187056037886No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGAA735.761641673243883No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCA705.524861878453039No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGAATCCTGTAGAACCAT120.9471191791633782No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGAATCCTGTAGAAC120.9471191791633782No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGAATCCTGTAGAAC90.7103393843725335No Hit
GCATCAATCATCACAGGGTGGCCAGCTCACTAATGGGAATCCTGTAGAAC80.6314127861089187No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC80.6314127861089187No Hit
GCATCAATCATCACAGGGTTGGCCAGCTACTAATGGGAATCCTGTAGAAC70.5524861878453038No Hit
GCATCAATCTCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.4735595895816891No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.4735595895816891No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.4735595895816891No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAAGGGAATCCTGTAGAAC50.3946329913180742No Hit
GCATCAATCATCACAGGGTTGGCCAGCCACTAATGGGAATCCTGTAGAAC50.3946329913180742No Hit
GCTCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.3946329913180742No Hit
GCATCAATCATCACAGGGTTGGCCGCTCACTAATGGGAATCCTGTAGAAC50.3946329913180742No Hit
CATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.31570639305445936No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACAATGGGAATCCTGTAGAAC40.31570639305445936No Hit
GATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.31570639305445936No Hit
GCATCAATCATCACAGGGTTGGCCGGCTCACTAATGGGAATCCTGTAGAA30.23677979479084454No Hit
GCATCAATCATCACAGGGTTGGCCACTCACTAATGGGAATCCTGTAGAAC30.23677979479084454No Hit
GCATCAATCATCACAGGGTTGGCAGCTCACTAATGGGAATCCTGTAGAAC30.23677979479084454No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTATGGGAATCCTGTAGAAC30.23677979479084454No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGGAATCCTGTAGAA30.23677979479084454No Hit
GCATCAATAATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA30.23677979479084454No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCCTAATGGGAATCCTGTAGAAC30.23677979479084454No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.23677979479084454No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCATAATGGGAATCCTGTAGAAC30.23677979479084454No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.23677979479084454No Hit
GCATAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.15785319652722968No Hit
ACGTTGCCTTTATCCTCTCCGACGTACATGATCTGAATCCTGTAGAACCA20.15785319652722968No Hit
ACGTTGCCTTTATCTCCCCGACGTACATGATCTGAATCCTGTAGAACCAT20.15785319652722968No Hit
GCATCAATCATCACAGGGTTGTCCAGCTCACTAATGGGAATCCTGTAGAA20.15785319652722968No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA20.15785319652722968Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.15785319652722968No Hit
ACGTTGCCTTTATACTCCCCGACGTACATGATCTGAATCCTGTAGAACCA20.15785319652722968No Hit
ACGTTGCCTTTATCCCCCCGACGTACATGATCTGAATCCTGTAGAACCAT20.15785319652722968No Hit
ACGTTGCCTTTATCCCCGACGTACATGATCTGAATCCTGTAGAACCATGT20.15785319652722968No Hit
GCATCAATCATCACAGGGTTGGCCAGTCCTAATGGGAATCCTGTAGAACC20.15785319652722968No Hit
GCATAATCATCACTGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.15785319652722968No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGAATCCTGTAGAAC20.15785319652722968No Hit
GCATCAATCATCACGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.15785319652722968No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCTAATGGGAATCCTGTAGAACC20.15785319652722968No Hit
GCATCAATCATCACCGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA20.15785319652722968No Hit
GCATCAATCACCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA20.15785319652722968No Hit
GCATCAATCATCACAGGGTTGGCCAGTCACTAATGGGAATCCTGTAGAAC20.15785319652722968No Hit
GCATCAATCATCCCAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA20.15785319652722968No Hit
ACGTTGCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCAT20.15785319652722968No Hit
GCACAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.15785319652722968No Hit
GCATCAATCATCACGGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGAA20.15785319652722968No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATAGGAATCCTGTAGAA20.15785319652722968No Hit
GCATCAATCATAACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA20.15785319652722968No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)