FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6437
Total Bases932.1 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG272442.317849930091654No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC123519.1859561907721No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAA98915.364300139816683No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCCGAGTTAGACCAA1111.7244057790896379No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTCGAGTTAGACCAAGTC921.4292372223085288No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG320.4971259903681839No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC280.43498524157216095No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG250.3883796799751437No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG210.32623893117912073No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG200.31070374398011497No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG190.2951685567811092No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTCGAGTTAGACCAAGTCT180.2796333695821035No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCGCACAGAAGCATATCCAG160.24856299518409194No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG150.23302780798508624No Hit
CCATTAGTGAGCTGGCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG150.23302780798508624No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCCGAGTTAGACCAA150.23302780798508624No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATGATGCCGAGTTAGACCAAG140.21749262078608048No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACGCAGAAGCATATCCAG130.20195743358707471No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG120.18642224638806898No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG120.18642224638806898No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCGG120.18642224638806898No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCGTATCCAG120.18642224638806898No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCCCACAGAAGCATATCCAG110.17088705918906325No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTATGCCGAGTTAGACCAAG110.17088705918906325No Hit
CCATTAGTGAGTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG110.17088705918906325No Hit
CCATTAGTGAGTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG110.17088705918906325No Hit
AGATCATTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG110.17088705918906325No Hit
AGATCATGTACGTCGGGGAGAGTACGGCGTCGATTCTAAAGTTGGGAGGA110.17088705918906325No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG100.15535187199005748No Hit
CCATTAGTGAGCTGGCCAACCAAGGTTGCTGGCTATACCTCGAGAAATCG90.13981668479105175No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCCGAGTTAGACCAAG90.13981668479105175No Hit
CCATTAGTAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG90.13981668479105175No Hit
CCATAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG90.13981668479105175No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGCCACACAGAAGCATATCCAG80.12428149759204597No Hit
AGATCATGTACGTCGGGGACGTCCACAGCGTCACACAGAAGCATATCCAG80.12428149759204597No Hit
AGATCATGTCGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG70.10874631039304024No Hit
CCATTAGTGGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG70.10874631039304024No Hit
CCATTAGTGAGCTGGCCAACCCTGTGAGATTGATGCCGAGTTAGACCAAG70.10874631039304024No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATAACCAG70.10874631039304024No Hit
CCATTGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG70.10874631039304024No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATGTCCAG70.10874631039304024No Hit
AGATCATGTACGTCGGGGACGTCCGCGGCGTCACACAGAAGCATATCCAG70.10874631039304024No Hit
AGCTCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG70.10874631039304024No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCCCGAGAAATC70.10874631039304024No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATACAG70.10874631039304024No Hit
AGATCAGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG70.10874631039304024No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATATTGATGCCGAGTTAGACCAAG70.10874631039304024No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATACCCAG70.10874631039304024No Hit
CCTTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG70.10874631039304024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)