FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6437
Total Bases923.5 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT231035.88628242970328No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT108716.88674848531925No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA94514.680751903060433No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA1031.600124281497592No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGGTCTTTGTAGAACCA921.4292372223085288No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG220.3417741183781265No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG220.3417741183781265No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG220.3417741183781265No Hit
CATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG200.31070374398011497No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG200.31070374398011497No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG190.2951685567811092No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGGTCTTTGTAGAACCAT190.2951685567811092No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT180.2796333695821035No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGGTCTTTGTAGAAC170.26409818238309773No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT150.23302780798508624No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC150.23302780798508624No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGGTGTCCCTCTT140.21749262078608048No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCAT130.20195743358707471No Hit
GCTCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG120.18642224638806898No Hit
ACGTTGCCTTTATCCTCCCAACTTTAGAATCGACGCCGTACTCTCCCCGA120.18642224638806898No Hit
GCATCAATCATCACAGGGTTGGCCACTCACTAATGGGGTCTTTGTAGAAC110.17088705918906325No Hit
GATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG110.17088705918906325No Hit
GCATAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC110.17088705918906325No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG110.17088705918906325No Hit
GCATCAATCATCACAGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG110.17088705918906325No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGCTGTCCCTCTT100.15535187199005748No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCACTT100.15535187199005748No Hit
GCATAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG100.15535187199005748No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAACTGGT100.15535187199005748No Hit
GCATCAATCTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG90.13981668479105175No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAACTCGCGAGATGTCCCTCTT90.13981668479105175No Hit
GCATCAATCATCACGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG90.13981668479105175No Hit
GCATCAATCAACACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT90.13981668479105175No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTC90.13981668479105175No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTA90.13981668479105175No Hit
GCATCAATCATCACAGGGGTGGACTCGAGAAATCGCGAGATGTCCCTCTT80.12428149759204597No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGCCCCTCTT80.12428149759204597No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGTCTTTGTAGAAC80.12428149759204597No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCAGGT80.12428149759204597No Hit
GCATCAATCATCACAGGGTTGACTCGAGAAATCGCGAGATGTCCCTCTTG80.12428149759204597No Hit
GCATCAATCATCACAGGGTTGGCCAGCTACTAATGGGGTCTTTGTAGAAC80.12428149759204597No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGAAGTCCCTCTT80.12428149759204597No Hit
GCATCAATCATCACAGGGTTGGACTCGGGAAATCGCGAGATGTCCCTCTT70.10874631039304024No Hit
GCATCAATCATCACAGGGTGGACTCGAGAAATCGCGAGATGTCCCTCTTG70.10874631039304024No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGACCCTCTT70.10874631039304024No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAAGGGGTCTTTGTAGAAC70.10874631039304024No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT70.10874631039304024No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC70.10874631039304024No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC70.10874631039304024No Hit
GCATCAATCATCCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT70.10874631039304024No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGACGTCCCTCTT70.10874631039304024No Hit
ACGTTGCCTATATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT70.10874631039304024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)