FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8840
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT397144.92081447963801No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT104111.77601809954751No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA98411.131221719457013No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA840.9502262443438914No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGGTCTTTGTAGAACCA770.8710407239819005No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG350.39592760180995473No Hit
ACGTTGCCTTTATCCTCCCAACTTTAGAATCGACGCCGTACTCTCCCCGA250.2828054298642534No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT240.27149321266968324No Hit
GATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG240.27149321266968324No Hit
GCATAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG230.2601809954751131No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG230.2601809954751131No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGCTGTCCCTCTT220.24886877828054296No Hit
GCATCAATCATCACCGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT220.24886877828054296No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCAT210.23755656108597284No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG210.23755656108597284No Hit
GCATCAATCTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG200.22624434389140274No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG190.2149321266968326No Hit
GCATCAATCATCACGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG190.2149321266968326No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGTCTTTGTAGAAC180.20361990950226247No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG170.19230769230769232No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCCCTT170.19230769230769232No Hit
GCATCAAACATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT160.18099547511312217No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGCCCCTCTT160.18099547511312217No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG150.16968325791855204No Hit
CATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG150.16968325791855204No Hit
GCATCAATCATCACAGGGTTGACTCGAGAAATCGCGAGATGTCCCTCTTG150.16968325791855204No Hit
GCATCAATCATCCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT150.16968325791855204No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG150.16968325791855204No Hit
GCATCAATCATCACAGGGTTGGACACGAGAAATCGCGAGATGTCCCTCTT150.16968325791855204No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAACCGCGAGATGTCCCTCTT140.1583710407239819No Hit
GCTCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG140.1583710407239819No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAACTCGCGAGATGTCCCTCTT140.1583710407239819No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCACTT140.1583710407239819No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGACGTCCCTCTT140.1583710407239819No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAGTCGCGAGATGTCCCTCTT130.14705882352941177No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGGTCTTTGTAGAAC130.14705882352941177No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGGGTCTTTGTAGAA120.13574660633484162No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGGTGTCCCTCTT120.13574660633484162No Hit
GCATCAATCATCACAGGGTTGGCTCGAGAAATCGCGAGATGTCCCTCTTG120.13574660633484162No Hit
GCATCAATCAACACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT120.13574660633484162No Hit
GCATCAATCACACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG120.13574660633484162No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGGTCTTTGTAGAACCAT110.12443438914027148No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT110.12443438914027148No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT110.12443438914027148No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT100.11312217194570137No Hit
GCATCAACATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG100.11312217194570137No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC100.11312217194570137No Hit
GCACAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG100.11312217194570137No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGACCCTCTT90.10180995475113123No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTGTT90.10180995475113123No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTA90.10180995475113123No Hit
GCATCAATCATCACAGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG90.10180995475113123No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGAAGTCCCTCTT90.10180995475113123No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)