FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3162
Total Bases455.3 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT159850.53763440860215No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA40112.681846932321317No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA551.739405439595193No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGGTCTTTGTAGAACCA321.0120177103099304No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT190.6008855154965212No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT180.5692599620493358No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG180.5692599620493358No Hit
ACGTTGCCTTTATCCTCCCAACTTTAGAATCGACGCCGTACTCTCCCCGA160.5060088551549652No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG120.3795066413662239No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGCGGGGCCCATTCGATCTGGT110.3478810879190386No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG110.3478810879190386No Hit
ACGTTGACTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT100.31625553447185323No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG100.31625553447185323No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG90.2846299810246679No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT90.2846299810246679No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATATGGT90.2846299810246679No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGGGTGGGGCCCATTCGATCTGGT80.2530044275774826No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGGTCTTTGTAGAAC80.2530044275774826No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAACTGGT70.2213788741302973No Hit
ACGTTGCCTATATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT70.2213788741302973No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT70.2213788741302973No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCAGGT70.2213788741302973No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGCTCTGGT60.18975332068311196No Hit
ACGTTGCCTTAATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT60.18975332068311196No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG60.18975332068311196No Hit
ACTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG50.15812776723592661No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATCCGATCTGGT50.15812776723592661No Hit
ACGTTGCCTTTATCCTCCCAGATCAAGGAGTGGGGCCCATTCGATCTGGT50.15812776723592661No Hit
GCATCAATCATCACAGGGTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC50.15812776723592661No Hit
GATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC50.15812776723592661No Hit
ACGTTGCCTTTATCCTCCCAGATCCTGGAGTGGGGCCCATTCGATCTGGT50.15812776723592661No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGCCCATTCGATCTGGTG50.15812776723592661No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC50.15812776723592661No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCACTCGATCTGGT50.15812776723592661No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGGTCTTTGTAGAACCAT50.15812776723592661No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGTCTTTGTAGAAC50.15812776723592661No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGGTCTGGT50.15812776723592661No Hit
ACGTAGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT50.15812776723592661No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTCGT50.15812776723592661No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCCGGT40.1265022137887413No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGTGCCCATTCGATCTGGT40.1265022137887413No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCAATCTGGT40.1265022137887413No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGGGTCTTTGTAGAA40.1265022137887413No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTATGGGGTCTTTGTAGAAC40.1265022137887413No Hit
ATGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT40.1265022137887413No Hit
GCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT40.1265022137887413No Hit
ACGTTGCCTTTATCCTCCCGATCCAGGAGTGGGGCCCATTCGATCTGGTG40.1265022137887413No Hit
ACGTTGCCTTTATCCTCCCACAGAGCCTTGGCATGCTCATAGAATTCGGG40.1265022137887413No Hit
GCATCAATCATCACAGGGTTTGGCCAGCTCACTAATGGGGTCTTTGTAGA40.1265022137887413No Hit
ACGTTGCCTTTATCATCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT40.1265022137887413No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCTCATTCGATCTGGT40.1265022137887413No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGTCCATTCGATCTGGT40.1265022137887413No Hit
ACGTTGCCTTTATCCTCCCAGACCCAGGAGTGGGGCCCATTCGATCTGGT40.1265022137887413No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGACCTGGT40.1265022137887413No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTAGATCTGGT40.1265022137887413No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTTGT40.1265022137887413No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)