FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7280
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG385452.93956043956044No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC101213.901098901098901No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAA90912.486263736263737No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCCGAGTTAGACCAA1321.8131868131868134No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTCGAGTTAGACCAAGTC320.43956043956043955No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG280.38461538461538464No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG270.37087912087912084No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC250.3434065934065934No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG250.3434065934065934No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG230.3159340659340659No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG220.30219780219780223No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG220.30219780219780223No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG210.2884615384615385No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGCCACACAGAAGCATATCCAG160.21978021978021978No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAGGCATATCCAG150.20604395604395606No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACGCAGAAGCATATCCAG150.20604395604395606No Hit
AGATCATGTACGTCGGGGACGTCCGCAGTGTCACACAGAAGCATATCCAG150.20604395604395606No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCCGAGTTAGACCAA140.19230769230769232No Hit
AGATCTGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG130.17857142857142858No Hit
AGATCATGTACGTCGGGGACGCCCGCAGCGTCACACAGAAGCATATCCAG120.16483516483516483No Hit
CCATTAGTGAGTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG120.16483516483516483No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCCCACAGAAGCATATCCAG110.15109890109890112No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGGAGCATATCCAG110.15109890109890112No Hit
AGATCATGACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG110.15109890109890112No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACGGAAGCATATCCAG100.13736263736263737No Hit
AGATCATGTACGTGGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG100.13736263736263737No Hit
AGATCATGTACGTCGGGGACGTCCGCGGCGTCACACAGAAGCATATCCAG100.13736263736263737No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATACAG100.13736263736263737No Hit
AGATATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG90.12362637362637362No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG90.12362637362637362No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCCGAGTTAGACCAAG90.12362637362637362No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCGG90.12362637362637362No Hit
CCATTAGTGAGCTGGCCAACCCTTGATGATTGATGCCGAGTTAGACCAAG90.12362637362637362No Hit
AGATCATTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG90.12362637362637362No Hit
AATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG90.12362637362637362No Hit
AGATCATGTACGTCGGGGACATCCGCAGCGTCACACAGAAGCATATCCAG80.10989010989010989No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGACACACAGAAGCATATCCAG80.10989010989010989No Hit
CCATTAGTGAGCTGGCAAACCCTGTGATGATTGATGCCGAGTTAGACCAA80.10989010989010989No Hit
AGATCATGTACGTCGGGGACGTCCACAGCGTCACACAGAAGCATATCCAG80.10989010989010989No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCGTATCCAG80.10989010989010989No Hit
CCATTGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG80.10989010989010989No Hit
AGATCACGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG80.10989010989010989No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATACCCAG80.10989010989010989No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)