FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2647
Total Bases383.4 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG88333.35851907820174No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAA59622.516055912353607No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC35113.26029467321496No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCCGAGTTAGACCAA782.9467321496033247No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTCGAGTTAGACCAAGTC762.871174914998111No Hit
AGATCATGTACGTCGGGGAGAGTACGGCGTCGATTCTAAAGTTGGGAGGA180.6800151114469211No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCCGAGTTAGACCAA120.4533434076312807No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC110.415564790328674No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG100.37778617302606726No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG100.37778617302606726No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG100.37778617302606726No Hit
CCATAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG90.34000755572346053No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTATGCCGAGTTAGACCAAG80.3022289384208538No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCCGAGTTAGACCAAG80.3022289384208538No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATGATGCCGAGTTAGACCAAG70.26445032111824707No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG70.26445032111824707No Hit
CCTTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG70.26445032111824707No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGATAGACCAA60.22667170381564036No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG60.22667170381564036No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG60.22667170381564036No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCCCACAGAAGCATATCCAG50.18889308651303363No Hit
AGATCATGTACGTCGGGGAGAGTACGGCGTCGATTCTAAAGTTGGTGGGA50.18889308651303363No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTCGAGTTAGACCAAGTCT50.18889308651303363No Hit
AGATCTGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG50.18889308651303363No Hit
CCATTAGTAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG50.18889308651303363No Hit
CCATTAGTGAGCTGGCCAACCCTTGATGATTGATGCCGAGTTAGACCAAG50.18889308651303363No Hit
CCATTAGTGAGCTGGCCAACCCTGGATGATTGATGCCGAGTTAGACCAAG50.18889308651303363No Hit
CCATTAGTGAGCTGGCCAACCCGTGATGATTGATGCCGAGTTAGACCAAG40.1511144692104269No Hit
CCATTAGTGGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG40.1511144692104269No Hit
AGATCATGTACGTCGGGGACGTCCGCGGCGTCACACAGAAGCATATCCAG40.1511144692104269No Hit
AGATCATGTACGTGGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG40.1511144692104269No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACGCAGAAGCATATCCAG40.1511144692104269No Hit
CCATTAGTGAGCTGGCCAAACCCTGTGATGATTGATGCCGAGTTAGACCA40.1511144692104269No Hit
CCATAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG40.1511144692104269No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG40.1511144692104269No Hit
AGATCATGACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG40.1511144692104269No Hit
AGATCATTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG40.1511144692104269No Hit
CCATTAGTGAGCTGGCCACCCTGTGATGATTGATGCCGAGTTAGACCAAG40.1511144692104269No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATCCGAGTTAGACCAAG40.1511144692104269No Hit
CCATTATGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG30.11333585190782018No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG30.11333585190782018No Hit
CCATTAGTGAGCTGGCCAACCAAGGTTGCTGGCTATACCTCGAGAAATCG30.11333585190782018No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATGATGCCGAGTTAGACCAAG30.11333585190782018No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCGCACAGAAGCATATCCAG30.11333585190782018No Hit
CCATTAGTGAGCTGGCCAACCCTGTGAGATTGATGCCGAGTTAGACCAAG30.11333585190782018No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACGGAAGCATATCCAG30.11333585190782018No Hit
AGATCATGTACGTCGGGGACGTCCACAGCGTCACACAGAAGCATATCCAG30.11333585190782018No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCGG30.11333585190782018No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCCATACCTCGAGAAATC30.11333585190782018No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTAGCTATACCTCGAGAAATC30.11333585190782018No Hit
CCATTAGTGAGTGGCCAAACCTGTGATGATTGATGCCGAGTTAGACCAAG30.11333585190782018No Hit
CCATTAGTGAGCTGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG30.11333585190782018No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCGAGTTAGACCAAG30.11333585190782018No Hit
AGATCATGTACGTCGGGGACGTCCGCAGAGTCACACAGAAGCATATCCAG30.11333585190782018No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG30.11333585190782018No Hit
AGCTCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG30.11333585190782018No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTAAGACCAA30.11333585190782018No Hit
AGATCATGTACGTCGGGGACGTCCGTAGCGTCACACAGAAGCATATCCAG30.11333585190782018No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGAAAGACCAA30.11333585190782018No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGACGCCGAGTTAGACCAA30.11333585190782018No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGTATATCCAG30.11333585190782018No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATATTGATGCCGAGTTAGACCAAG30.11333585190782018No Hit
CCATTAGTGAGCTGGCCAAACCCGAGAACCAGTTCAGAGTGGACTACATT30.11333585190782018No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATACCCAG30.11333585190782018No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)