FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3729
Total Bases537.2 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT185949.852507374631266No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA51313.757039420756234No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA651.743094663448646No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGGTCTTTGTAGAACCA501.3408420488066506No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT240.6436041834271923No Hit
ACGTTGCCTTTATCCTCCCAACTTTAGAATCGACGCCGTACTCTCCCCGA200.5363368195226602No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT190.5095199785465273No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG180.4827031375703942No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGTCTTTGTAGAAC170.45588629659426116No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG160.4290694556181282No Hit
ACGTTGCCTTTAACCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT120.32180209171359614No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT110.2949852507374631No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG110.2949852507374631No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG100.2681684097613301No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGA100.2681684097613301No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCAGGT100.2681684097613301No Hit
ACTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG90.2413515687851971No Hit
ACGTTGACTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT90.2413515687851971No Hit
ACGTTGCCTATATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT90.2413515687851971No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGTTCAACCGGAGTTACCTGAGCCC90.2413515687851971No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGACCATTCGATCTGGT90.2413515687851971No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGCTCTGGT90.2413515687851971No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATATGGT90.2413515687851971No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG90.2413515687851971No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGGTCTTTGTAGAAC80.2145347278090641No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG80.2145347278090641No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC80.2145347278090641No Hit
ACGGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT70.18771788683293109No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCACTCGATCTGGT70.18771788683293109No Hit
ACGTTGCCTTAATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT70.18771788683293109No Hit
GCATAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC60.16090104585679807No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAACTGGT60.16090104585679807No Hit
ACGTTGCCTTTATCCTCCCAGGTCCAGGAGTGGGGCCCATTCGATCTGGT60.16090104585679807No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGGTCTTTGTAGAACCAT60.16090104585679807No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATACGATCTGGT60.16090104585679807No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTCGT60.16090104585679807No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGGGTGGGGCCCATTCGATCTGGT50.13408420488066505No Hit
GCATCAATCATCACCGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA50.13408420488066505No Hit
ACGTTGCCTTTATCATCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT50.13408420488066505No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCTCATTCGATCTGGT50.13408420488066505No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT50.13408420488066505No Hit
GCTCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC50.13408420488066505No Hit
ACGTTGCCTTTATCCTCCCAGACCCAGGAGTGGGGCCCATTCGATCTGGT50.13408420488066505No Hit
ACGTAGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT50.13408420488066505No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCCGGT40.10726736390453205No Hit
GCATCAATCATCACAGTTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC40.10726736390453205No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC40.10726736390453205No Hit
ACGTTGCCTTTGTCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT40.10726736390453205No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATCCGATCTGGT40.10726736390453205No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGCGGGGCCCATTCGATCTGGT40.10726736390453205No Hit
ACGTTGCCTTTATCCTCCCAGATCAAGGAGTGGGGCCCATTCGATCTGGT40.10726736390453205No Hit
ACGTTGCCTTTATCCCCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT40.10726736390453205No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACAATGGGGTCTTTGTAGAAC40.10726736390453205No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGACCCATTCGATCTGGT40.10726736390453205No Hit
ACGTTGCCTTTATCCTCCCAGATCCGGGAGTGGGGCCCATTCGATCTGGT40.10726736390453205No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGTGGCCCATTCGATCTGGT40.10726736390453205No Hit
ACGTTGCCTTTATCCTCCCACCAACTTTAGAATCGACGCCGTACTCTCCC40.10726736390453205No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC40.10726736390453205No Hit
ACATTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT40.10726736390453205No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCATAATGGGGTCTTTGTAGAAC40.10726736390453205No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTTGT40.10726736390453205No Hit
ACGTTGCCTTTTCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG40.10726736390453205No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAAGGGGTCTTTGTAGAAC40.10726736390453205No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)