FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4625
Total Bases668.6 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT202843.84864864864865No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA99521.513513513513512No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA1433.091891891891892No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGGTCTTTGTAGAACCA731.5783783783783782No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG220.4756756756756757No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT200.43243243243243246No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG160.34594594594594597No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATATGGT160.34594594594594597No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCAGGT150.3243243243243243No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGTCTTTGTAGAAC140.3027027027027027No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG130.2810810810810811No Hit
ACGTTGCCTTTATCCTCCCAACTTTAGAATCGACGCCGTACTCTCCCCGA120.2594594594594595No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGGTCTTTGTAGAAC120.2594594594594595No Hit
ACGTTGCCTTTAACCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT110.23783783783783785No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC110.23783783783783785No Hit
ACGTTGCCTATATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT110.23783783783783785No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG100.21621621621621623No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT100.21621621621621623No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC100.21621621621621623No Hit
GCATCAATCATCACAGGGTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC90.19459459459459458No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT90.19459459459459458No Hit
GCATCAATCATCACAGGGTTGGCCAGCCACTAATGGGGTCTTTGTAGAAC90.19459459459459458No Hit
ACTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG90.19459459459459458No Hit
GCATCAATCATCACAGGGTTGGCAGCTCACTAATGGGGTCTTTGTAGAAC80.17297297297297298No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACAATGGGGTCTTTGTAGAAC70.15135135135135136No Hit
ACGTTGACTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT70.15135135135135136No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG70.15135135135135136No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATCCGATCTGGT70.15135135135135136No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGCCCATTCGATCTGGTG70.15135135135135136No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC70.15135135135135136No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCAATCTGGT60.12972972972972974No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGGGTCTTTGTAGAA60.12972972972972974No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAATCGATCTGGT60.12972972972972974No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCATAATGGGGTCTTTGTAGAAC60.12972972972972974No Hit
ACGTTGCCTTTTCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG60.12972972972972974No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG60.12972972972972974No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAACTGGT60.12972972972972974No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGGTCTGGT60.12972972972972974No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCTATCTGGT50.10810810810810811No Hit
GCATCAATCATCACAGGGTTGGCCAGCTAACTAATGGGGTCTTTGTAGAA50.10810810810810811No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGTGGCCCATTCGATCTGGT50.10810810810810811No Hit
GCATCAATCATCACGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC50.10810810810810811No Hit
GCATCAATCATCACAGGGTTGACCAGCTCACTAATGGGGTCTTTGTAGAA50.10810810810810811No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGAGGGGCCCATTCGATCTGGT50.10810810810810811No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGGTCTTTGTAGAACCAT50.10810810810810811No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC50.10810810810810811No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTATGGGGTCTTTGTAGAAC50.10810810810810811No Hit
ACGTTGCCTTTATCCTCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG50.10810810810810811No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGACCCATTCGATCTGGT50.10810810810810811No Hit
ACGTTGCCTTTATCCTCCCAGATCCGGGAGTGGGGCCCATTCGATCTGGT50.10810810810810811No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT50.10810810810810811No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT50.10810810810810811No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGA50.10810810810810811No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGCTCTGGT50.10810810810810811No Hit
ACGTAGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT50.10810810810810811No Hit
GCATCAATCACACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC50.10810810810810811No Hit
ACGATGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT50.10810810810810811No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTCGT50.10810810810810811No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)