FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9156
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG479952.41371778069026No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC131414.35124508519004No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAA98110.714285714285714No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCCGAGTTAGACCAA1171.2778505897771952No Hit
AGATCATGTACGTCGGGGACGTCCACAGCGTCACACAGAAGCATATCCAG1081.1795543905635648No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTCGAGTTAGACCAAGTC720.7863695937090431No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG380.4150283966797728No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG370.4041065967671472No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG310.3385757972913936No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC300.327653997378768No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG270.2948885976408912No Hit
AGATATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG270.2948885976408912No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG270.2948885976408912No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCCCACAGAAGCATATCCAG260.2839667977282656No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG240.2621231979030144No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCGTATCCAG220.2402795980777632No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG220.2402795980777632No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG210.22935779816513763No Hit
AGATCATGTACGTCGGGGACGCCCGCAGCGTCACACAGAAGCATATCCAG190.2075141983398864No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCCGAGTTAGACCAA180.19659239842726078No Hit
AGATCTGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG170.18567059851463522No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCGG170.18567059851463522No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG170.18567059851463522No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACGGAAGCATATCCAG160.17474879860200962No Hit
AGTCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG160.17474879860200962No Hit
AGATCATGTACGTGGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG150.163826998689384No Hit
AGATCATGTACGTCGGGGAGAGTACGGCGTCGATTCTAAAGTTGGGAGGA150.163826998689384No Hit
CCATTAGTGAGTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG140.1529051987767584No Hit
AGATCATGTACGTCGGGGACGTCCGCGGCGTCACACAGAAGCATATCCAG140.1529051987767584No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCGCACAGAAGCATATCCAG140.1529051987767584No Hit
AGATCAGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG130.1419833988641328No Hit
AGCTCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG120.1310615989515072No Hit
AGATCATTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG120.1310615989515072No Hit
GATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG110.1201397990388816No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCCGAGTTAGACCAAG110.1201397990388816No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGCCACACAGAAGCATATCCAG110.1201397990388816No Hit
CCATTAGTGAGCTGGCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG110.1201397990388816No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACGCAGAAGCATATCCAG110.1201397990388816No Hit
AGATCATGTACGTCGGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAG110.1201397990388816No Hit
AGATCATGACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG110.1201397990388816No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATACAG110.1201397990388816No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAACATATCCAG100.109217999126256No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGGAGCATATCCAG100.109217999126256No Hit
AGATCATGTACGTCGGGGACGTCTGCAGCGTCACACAGAAGCATATCCAG100.109217999126256No Hit
AATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG100.109217999126256No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)