FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7483
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG259234.638513964987304No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC175923.506614993986368No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAA129917.359347855138314No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCCGAGTTAGACCAA1552.071361753307497No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTCGAGTTAGACCAAGTC1181.576907657356675No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC370.49445409595082185No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG350.4677268475210477No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG240.32072698115728987No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTCGAGTTAGACCAAGTCT230.3073633569424028No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG220.2939997327275157No Hit
AGATCATGTACGTCGGGGAGAGTACGGCGTCGATTCTAAAGTTGGGAGGA220.2939997327275157No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG180.2405452358679674No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG160.21381798743819325No Hit
CCATTAGTGAGCTGGCCAACCAAGGTTGCTGGCTATACCTCGAGAAATCG150.20045436322330615No Hit
AGATCTGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG150.20045436322330615No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG150.20045436322330615No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCCGAGTTAGACCAA150.20045436322330615No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG130.173727114793532No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAGGCATATCCAG130.173727114793532No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGCCACACAGAAGCATATCCAG130.173727114793532No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCCCACAGAAGCATATCCAG120.16036349057864493No Hit
CCATTAGTGAGCTGGCCAACCTGTGATGATTGATGCCGAGTTAGACCAAG120.16036349057864493No Hit
CCATTAGTGAGCTGGCAAACCCTGTGATGATTGATGCCGAGTTAGACCAA120.16036349057864493No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG120.16036349057864493No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTATGCCGAGTTAGACCAAG110.14699986636375784No Hit
CCATTAGTGAGTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG110.14699986636375784No Hit
AGATCATGTACGTCGGGGACGTCCACAGCGTCACACAGAAGCATATCCAG110.14699986636375784No Hit
CCATTAGTAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG110.14699986636375784No Hit
CCATTAGGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG110.14699986636375784No Hit
CCATTATGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG100.13363624214887077No Hit
CCTTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG100.13363624214887077No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG100.13363624214887077No Hit
CCATTATGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG100.13363624214887077No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACGCAGAAGCATATCCAG100.13363624214887077No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATATTGATGCCGAGTTAGACCAAG100.13363624214887077No Hit
AGATATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG90.1202726179339837No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGACACACAGAAGCATATCCAG90.1202726179339837No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCCGAGTTAGACCAAG90.1202726179339837No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCCGGCTATACCTCGAGAAATC90.1202726179339837No Hit
CCATTAGTGAGCGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG90.1202726179339837No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCGG90.1202726179339837No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCGTATCCAG90.1202726179339837No Hit
CCATAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG90.1202726179339837No Hit
CCATTGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG90.1202726179339837No Hit
AGATCATGTACGTGGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG80.10690899371909662No Hit
AGATCATGTACGTCGGGGACATCCGCAGCGTCACACAGAAGCATATCCAG80.10690899371909662No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTAAGACCAA80.10690899371909662No Hit
CCATTAGTGAGCTGGCCAACCCTGGATGATTGATGCCGAGTTAGACCAAG80.10690899371909662No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCGCACAGAAGCATATCCAG80.10690899371909662No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCAGATCCAG80.10690899371909662No Hit
CCATTAGTGACTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG80.10690899371909662No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACAAA80.10690899371909662No Hit
CCATTAGTGAGTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG80.10690899371909662No Hit
AGATCATGTACGTCGGGGACGTCCGCAGTGTCACACAGAAGCATATCCAG80.10690899371909662No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)