FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5119
Total Bases737.3 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT193737.839421762062905No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA89817.54248876733737No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT2755.372142996679039No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTT2094.08282867747607No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGGTCTTTGTAGAACCA1082.109787067786677No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA1052.0511818714592693No Hit
ACGTTGCCTTTATCCTCCCAACTTTAGAATCGACGCCGTACTCTCCCCGA320.6251220941590154No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG230.4493065051767923No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT190.3711662434069154No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT180.35163117796444615No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG130.25395585075210003No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG130.25395585075210003No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCAGGT130.25395585075210003No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATATGGT130.25395585075210003No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT110.21488571986716157No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGTCTTTGTAGAAC110.21488571986716157No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG100.19535065442469235No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG90.17581558898222308No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC90.17581558898222308No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGGTCTTTGTAGAAC90.17581558898222308No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGGTCTTTGTAGAACCAT80.15628052353975386No Hit
ACGTTGCCTTAATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT80.15628052353975386No Hit
ACGTAGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT80.15628052353975386No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC80.15628052353975386No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCTATCTGGT70.1367454580972846No Hit
GCATCAATCATCACAGGGTTGGCCAGCCACTAATGGGGTCTTTGTAGAAC70.1367454580972846No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCATAATGGGGTCTTTGTAGAAC70.1367454580972846No Hit
ACTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG70.1367454580972846No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGCTCTGGT70.1367454580972846No Hit
GCATCAATCATCACAGGGTTGGCCACTCACTAATGGGGTCTTTGTAGAAC60.1172103926548154No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGGGTGGGGCCCATTCGATCTGGT60.1172103926548154No Hit
ACGTTGCCTTTAACCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT60.1172103926548154No Hit
ACGTTGCCTTTATCCTCCCAGACCCAGGAGTGGGGCCCATTCGATCTGGT60.1172103926548154No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG60.1172103926548154No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATCCGATCTGGT60.1172103926548154No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAACTGGT60.1172103926548154No Hit
ACGTTGCCTTTAGCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT60.1172103926548154No Hit
ACGTTGCCTTTATCCTCCCACCAACTTTAGAATCGACGCCGTACTCTCCC60.1172103926548154No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT60.1172103926548154No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)