FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8976
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT415946.33467023172905No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT101311.285650623885918No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA100411.185383244206774No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA1171.303475935828877No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGGTCTTTGTAGAACCA941.0472370766488415No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG420.4679144385026738No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT370.4122103386809269No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCAGGT240.267379679144385No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG230.25623885918003564No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGTCTTTGTAGAAC230.25623885918003564No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG230.25623885918003564No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG220.24509803921568626No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG210.2339572192513369No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT190.21167557932263814No Hit
ACTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG190.21167557932263814No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAACTGGT180.2005347593582888No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGCTCTGGT160.17825311942959002No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT150.16711229946524064No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGCGGGGCCCATTCGATCTGGT140.15597147950089127No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGA140.15597147950089127No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCACTCGATCTGGT130.1448306595365419No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATCCGATCTGGT130.1448306595365419No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT130.1448306595365419No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGGTCTGGT130.1448306595365419No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATATGGT130.1448306595365419No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCGTTCGATCTGGT120.1336898395721925No Hit
ACGTTGCCTTTATCCTCCCAACTTTAGAATCGACGCCGTACTCTCCCCGA120.1336898395721925No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG120.1336898395721925No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGGTCTTTGTAGAAC120.1336898395721925No Hit
ACGTTGCCTTTATCCTCCCAGATCCGGGAGTGGGGCCCATTCGATCTGGT120.1336898395721925No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGGGTGGGGCCCATTCGATCTGGT110.12254901960784313No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGACCATTCGATCTGGT110.12254901960784313No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGACCTGGT110.12254901960784313No Hit
ACGATGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT110.12254901960784313No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC100.11140819964349377No Hit
ACGTTGCCTTTATCCTCCCAGACCCAGGAGTGGGGCCCATTCGATCTGGT100.11140819964349377No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC100.11140819964349377No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTT100.11140819964349377No Hit
ACGTTGCCTTTATCCTCCCAGTTCCAGGAGTGGGGCCCATTCGATCTGGT100.11140819964349377No Hit
ACGTTGCCTTTATCCTCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG100.11140819964349377No Hit
ACGTTGCCTATATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT100.11140819964349377No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGC100.11140819964349377No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGG100.11140819964349377No Hit
ACGTAGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT100.11140819964349377No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTTGT100.11140819964349377No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT90.1002673796791444No Hit
ACGTTGCCTTTAACCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT90.1002673796791444No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGGTCTTTGTAGAACCAT90.1002673796791444No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCCGGT90.1002673796791444No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGTGCCCATTCGATCTGGT90.1002673796791444No Hit
ACGGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT90.1002673796791444No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGAGGCCCATTCGATCTGGT90.1002673796791444No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)