FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7229
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG253235.025591368100706No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC153921.289251625397704No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAA130818.0937889057961No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCCGAGTTAGACCAA1692.3378060589293126No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTCGAGTTAGACCAAGTC1251.7291464932909117No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC390.5394937059067644No Hit
AGATCATGTACGTCGGGGAGAGTACGGCGTCGATTCTAAAGTTGGGAGGA320.44266150228247336No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG300.41499515838981876No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG260.3596624706045096No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG220.3043297828192004No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG210.29049661087287315No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCCGAGTTAGACCAA210.29049661087287315No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG180.24899709503389128No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG170.23516392308756398No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGCCACACAGAAGCATATCCAG140.19366440724858208No Hit
CCATTAGTGGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG130.1798312353022548No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG130.1798312353022548No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACGCAGAAGCATATCCAG130.1798312353022548No Hit
CCATTATGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG120.1659980633559275No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG120.1659980633559275No Hit
AGATCATGTACGTCGGGGACGTCCGCAGTGTCACACAGAAGCATATCCAG120.1659980633559275No Hit
CCATAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG110.1521648914096002No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTATGCCGAGTTAGACCAAG110.1521648914096002No Hit
AGATCATGTACGTCGGGGACGCCCGCAGCGTCACACAGAAGCATATCCAG110.1521648914096002No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATGATGCCGAGTTAGACCAAG110.1521648914096002No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATATTGATGCCGAGTTAGACCAAG110.1521648914096002No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTCGAGTTAGACCAAGTCT100.1383317194632729No Hit
CCATTAGTGAGTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG100.1383317194632729No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCGCACAGAAGCATATCCAG100.1383317194632729No Hit
AGATATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG90.12449854751694564No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACGGAAGCATATCCAG90.12449854751694564No Hit
CCATTAGTGAGCTGGCCACCCTGTGATGATTGATGCCGAGTTAGACCAAG90.12449854751694564No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG80.11066537557061834No Hit
AGTCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG80.11066537557061834No Hit
CCATTAGTGAGCTGGCCAAACCCTGTGATGATTGATGCCGAGTTAGACCA80.11066537557061834No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCCGAGTTAGACCAAG80.11066537557061834No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGGAGCATATCCAG80.11066537557061834No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCCGGCTATACCTCGAGAAATC80.11066537557061834No Hit
CCATTAGTGAGCTGGCCAACCCTGGATGATTGATGCCGAGTTAGACCAAG80.11066537557061834No Hit
CCATTAGTGGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG80.11066537557061834No Hit
AGATCATGTACGTCGGGGACGTCCACAGCGTCACACAGAAGCATATCCAG80.11066537557061834No Hit
AGATCATGTACGTCGGGGACGTCTGCAGCGTCACACAGAAGCATATCCAG80.11066537557061834No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)