FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5404
Total Bases780.1 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT183934.030347890451516No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA109420.244263508512216No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT68112.601776461880087No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA1021.8874907475943745No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGGTCTTTGTAGAACCA861.5914137675795705No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG210.38860103626943004No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG210.38860103626943004No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT190.3515914137675796No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG170.3145817912657291No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGTCTTTGTAGAAC160.2960769800148038No Hit
ACGTTGCCTTTATCCTCCCAACTTTAGAATCGACGCCGTACTCTCCCCGA140.2590673575129534No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTATGGGGTCTTTGTAGAAC140.2590673575129534No Hit
GCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT110.20355292376017764No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT100.1850481125092524No Hit
GCATAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC100.1850481125092524No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATATGGT100.1850481125092524No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC100.1850481125092524No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG90.16654330125832717No Hit
GCATCAATCATCACAGGGTTGGCCAGCCACTAATGGGGTCTTTGTAGAAC90.16654330125832717No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC90.16654330125832717No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGGTCTTTGTAGAAC90.16654330125832717No Hit
ACGTTGCCTTTATCCTCCCACCAACTTTAGAATCGACGCCGTACTCTCCC90.16654330125832717No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCGTTCGATCTGGT80.1480384900074019No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGGGTGGGGCCCATTCGATCTGGT80.1480384900074019No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC80.1480384900074019No Hit
ACGTTGCCTTTTCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG80.1480384900074019No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAACTGGT80.1480384900074019No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGACCATTCGATCTGGT80.1480384900074019No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGG80.1480384900074019No Hit
GCATCAATCATCACAGGGTTGGCCACTCACTAATGGGGTCTTTGTAGAAC70.1295336787564767No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACAATGGGGTCTTTGTAGAAC70.1295336787564767No Hit
ACGTTGCCTTTAACCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT70.1295336787564767No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCATAATGGGGTCTTTGTAGAAC70.1295336787564767No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC70.1295336787564767No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC70.1295336787564767No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGCGGGGCCCATTCGATCTGGT70.1295336787564767No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGAGCCCATTCGATCTGGT70.1295336787564767No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT60.11102886750555144No Hit
ACGTTGACTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT60.11102886750555144No Hit
GCATCAATCATCACAGGGTTTGGCCAGCTCACTAATGGGGTCTTTGTAGA60.11102886750555144No Hit
ACGTTGCCTTTATCCTCCCAGACCCAGGAGTGGGGCCCATTCGATCTGGT60.11102886750555144No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG60.11102886750555144No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCAGGT60.11102886750555144No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGTGCCCATTCGATCTGGT60.11102886750555144No Hit
GCATCAATCATCACAGGGTTGGACAGCTCACTAATGGGGTCTTTGTAGAA60.11102886750555144No Hit
GCATCAATCATCACAGGGTTGGCCAGCTACTAATGGGGTCTTTGTAGAAC60.11102886750555144No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGACCCATTCGATCTGGT60.11102886750555144No Hit
GATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC60.11102886750555144No Hit
GCATCAATCATCACAGGGTTGGCAGCTCACTAATGGGGTCTTTGTAGAAC60.11102886750555144No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)