FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2414
Total Bases349.7 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC59

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG74330.778790389395194No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAA61725.559237779618886No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC33513.87738193869097No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCCGAGTTAGACCAA843.47970173985087No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTCGAGTTAGACCAAGTC813.3554266777133392No Hit
AGATCATGTACGTCGGGGAGAGTACGGCGTCGATTCTAAAGTTGGGAGGA220.9113504556752278No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG160.6628003314001657No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCCGAGTTAGACCAA130.5385252692626347No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTCGAGTTAGACCAAGTCT100.4142502071251036No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG80.33140016570008285No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC70.2899751449875725No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG70.2899751449875725No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTATGCCGAGTTAGACCAAG60.24855012427506215No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCCCACAGAAGCATATCCAG50.2071251035625518No Hit
CCATTATGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG50.2071251035625518No Hit
AGATCATGTACGTCGGGGAGAGTACGGCGTCGATTCTAAAGTTGGTGGGA50.2071251035625518No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCGCACAGAAGCATATCCAG50.2071251035625518No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG50.2071251035625518No Hit
CCATTAGTGAGCTGGCCAACCCTTGATGATTGATGCCGAGTTAGACCAAG50.2071251035625518No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATGATGCCGAGTTAGACCAAG50.2071251035625518No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCGTATCCAG50.2071251035625518No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG50.2071251035625518No Hit
CCATTAGTGAGCTGGCCACCCTGTGATGATTGATGCCGAGTTAGACCAAG50.2071251035625518No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG50.2071251035625518No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG50.2071251035625518No Hit
CCATTAGGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG40.16570008285004142No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGATATC40.16570008285004142No Hit
AGATCATGTACGTCGGGGACGTCCACAGCGTCACACAGAAGCATATCCAG40.16570008285004142No Hit
AGATCTGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG40.16570008285004142No Hit
CCATTAGTGAGCTGGCCAAACCCTGTGATGATTGATGCCGAGTTAGACCA40.16570008285004142No Hit
CCATTAGTGAGTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG40.16570008285004142No Hit
CCATTAGTGAGCTGGCCAACCCTGGATGATTGATGCCGAGTTAGACCAAG40.16570008285004142No Hit
CCATTAGTGAGCGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG40.16570008285004142No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATCCGAGTTAGACCAAG40.16570008285004142No Hit
AGATCAGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG40.16570008285004142No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATATTGATGCCGAGTTAGACCAAG40.16570008285004142No Hit
CCTTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG40.16570008285004142No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGATAGACCAA30.12427506213753108No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG30.12427506213753108No Hit
AGATCATGTCGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG30.12427506213753108No Hit
CCATTAGTGAGCTGGCCAACCAAGGTTGCTGGCTATACCTCGAGAAATCG30.12427506213753108No Hit
AGATCATGTACGTCGGGGACGTCCGCGGCGTCACACAGAAGCATATCCAG30.12427506213753108No Hit
CCATTAGTGAGCTGGCCAACCCTGAGATGATTGATGCCGAGTTAGACCAA30.12427506213753108No Hit
CCATTAGTGAGCTGGCAAACCCTGTGATGATTGATGCCGAGTTAGACCAA30.12427506213753108No Hit
AGATCATGTACGTGGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG30.12427506213753108No Hit
AGTCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG30.12427506213753108No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCGG30.12427506213753108No Hit
CCATTAGGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG30.12427506213753108No Hit
CCATAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG30.12427506213753108No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGAGCCGAGTTAGACCAAG30.12427506213753108No Hit
AGATCATGTACGTCGGAGACGTCCGCAGCGTCACACAGAAGCATATCCAG30.12427506213753108No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCCGAGTTAGACCAAG30.12427506213753108No Hit
AGATCATGTACGTCGGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAG30.12427506213753108No Hit
CCATTAGTGAGCTGGCCAAACCAAGGATGCTGGCTATACCTCGAGAAATC30.12427506213753108No Hit
CCATTGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG30.12427506213753108No Hit
CCATTAGTGACTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG30.12427506213753108No Hit
AGATCATGTACGTCGGGGACGTCCGCAGTGTCACACAGAAGCATATCCAG30.12427506213753108No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)