FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3869
Total Bases557.5 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT175745.412251227707415No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA71518.48022744895322No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA852.196950116309124No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGGTCTTTGTAGAACCA631.6283277332644095No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT210.5427759110881365No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT170.43939002326182475No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG170.43939002326182475No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGTCTTTGTAGAAC150.3876970793486689No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC130.33600413543551305No Hit
ACGTTGCCTTTATCCTCCCAACTTTAGAATCGACGCCGTACTCTCCCCGA120.31015766347893514No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCACTCGATCTGGT120.31015766347893514No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG120.31015766347893514No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGGTCTTTGTAGAAC100.25846471956577927No Hit
ACTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG90.23261824760920136No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAACTGGT90.23261824760920136No Hit
ACGTTGACTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT90.23261824760920136No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT90.23261824760920136No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGCTCTGGT90.23261824760920136No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC90.23261824760920136No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC90.23261824760920136No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC80.20677177565262342No Hit
ACGTAGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT80.20677177565262342No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATATGGT80.20677177565262342No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT70.18092530369604548No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGACCATTCGATCTGGT70.18092530369604548No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG70.18092530369604548No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG70.18092530369604548No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTCGT70.18092530369604548No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCTATCTGGT60.15507883173946757No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG60.15507883173946757No Hit
GCATCAATCATCACAGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC60.15507883173946757No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGACCCATTCGATCTGGT60.15507883173946757No Hit
ACGTTGCCTATATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT60.15507883173946757No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCATAATGGGGTCTTTGTAGAAC60.15507883173946757No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG60.15507883173946757No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCAGGT60.15507883173946757No Hit
GCATCAATCATCCCAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA50.12923235978288963No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTATGGGGTCTTTGTAGAAC50.12923235978288963No Hit
GCATCAATCATCACAGGGTTGGCCAGCTACTAATGGGGTCTTTGTAGAAC50.12923235978288963No Hit
ACGTTGCCTTTATCCTCCCGATCCAGGAGTGGGGCCCATTCGATCTGGTG50.12923235978288963No Hit
ACGTTGCCTTTAACCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT50.12923235978288963No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCTCATTCGATCTGGT50.12923235978288963No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGCCCATTCGATCTGGTG50.12923235978288963No Hit
CATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC50.12923235978288963No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGAGGGGCCCATTCGATCTGGT50.12923235978288963No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGGTCTTTGTAGAACCAT50.12923235978288963No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGACCTGGT50.12923235978288963No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTAGATCTGGT50.12923235978288963No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATACGATCTGGT50.12923235978288963No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAAGGGGTCTTTGTAGAAC50.12923235978288963No Hit
ACGTTGCCTTTATCCTCCCAGATCCCGGAGTGGGGCCCATTCGATCTGGT40.10338588782631171No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGGGTGGGGCCCATTCGATCTGGT40.10338588782631171No Hit
GCATAAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA40.10338588782631171No Hit
ACGTTTCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT40.10338588782631171No Hit
GCATCAATCATCACCGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA40.10338588782631171No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGAT40.10338588782631171No Hit
GATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC40.10338588782631171No Hit
ACGTTGCCTTTATCCTCCCAGATCCGGGAGTGGGGCCCATTCGATCTGGT40.10338588782631171No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGCT40.10338588782631171No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCTTTCGATCTGGT40.10338588782631171No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCGATTCGATCTGGT40.10338588782631171No Hit
GCATCAATCATCACAGGGTTGGCAGCTCACTAATGGGGTCTTTGTAGAAC40.10338588782631171No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGCGCCCATTCGATCTGGT40.10338588782631171No Hit
ACGTTCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG40.10338588782631171No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)