FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8170
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT293235.88739290085679No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT126915.532435740514074No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA122915.042839657282741No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA1722.1052631578947367No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGGTCTTTGTAGAACCA1391.701346389228886No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGGTCTTTGTAGAACCAT240.2937576499388005No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGGTCTTTGTAGAAC230.28151774785801714No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGTCTTTGTAGAAC210.25703794369645044No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG210.25703794369645044No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG210.25703794369645044No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG180.2203182374541004No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG170.208078335373317No Hit
GCATCAATCATCACGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG170.208078335373317No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC170.208078335373317No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT160.19583843329253367No Hit
GCATCAATCTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG160.19583843329253367No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTA150.18359853121175032No Hit
ACGTTGCCTTTATCCTCCCAACTTTAGAATCGACGCCGTACTCTCCCCGA140.17135862913096694No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAACTCGCGAGATGTCCCTCTT140.17135862913096694No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGCTGTCCCTCTT130.1591187270501836No Hit
GATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG130.1591187270501836No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTC130.1591187270501836No Hit
GCTCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG120.14687882496940025No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGCCCCTCTT120.14687882496940025No Hit
GCATCAATCATCACAGGGTTGGCCAGCCACTAATGGGGTCTTTGTAGAAC120.14687882496940025No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCAT120.14687882496940025No Hit
GCATCAATCATCACAGGGTTGGACTCGAGGAATCGCGAGATGTCCCTCTT120.14687882496940025No Hit
GCATAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG120.14687882496940025No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG120.14687882496940025No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT110.1346389228886169No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAGTCGCGAGATGTCCCTCTT110.1346389228886169No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG110.1346389228886169No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT110.1346389228886169No Hit
GCATCAATCATCACAGGGTTGGCAGCTCACTAATGGGGTCTTTGTAGAAC110.1346389228886169No Hit
GCATCAATCATCACAGGGTTGGACTCGGGAAATCGCGAGATGTCCCTCTT100.12239902080783352No Hit
GCATCAATCATCACAGGGTGGACTCGAGAAATCGCGAGATGTCCCTCTTG100.12239902080783352No Hit
GCATCAAACATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT100.12239902080783352No Hit
GCATCAATCATCACAGGGTTGGCTCGAGAAATCGCGAGATGTCCCTCTTG100.12239902080783352No Hit
CATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG100.12239902080783352No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGGGATGTCCCTCTT100.12239902080783352No Hit
GCATCAATCATCACAGGGTTGACTCGAGAAATCGCGAGATGTCCCTCTTG100.12239902080783352No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG100.12239902080783352No Hit
GCATCAATCATCACAGGGTTGGACCCGAGAAATCGCGAGATGTCCCTCTT90.1101591187270502No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGGGTCTTTGTAGAA90.1101591187270502No Hit
GCATCAATCATCACGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC90.1101591187270502No Hit
GCATCAATCATCACAGGGTTGGCCAGCTACTAATGGGGTCTTTGTAGAAC90.1101591187270502No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT90.1101591187270502No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)