FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5731
Total Bases829.7 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG165828.93037864247077No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC125621.91589600418775No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAA122221.322631303437444No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCCGAGTTAGACCAA1763.071017274472169No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTCGAGTTAGACCAAGTC1292.2509160704938056No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG250.43622404466934217No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCCGAGTTAGACCAA230.4013261210957948No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG220.3838771593090211No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTCGAGTTAGACCAAGTCT190.33153027394870005No Hit
AGATCATGTACGTCGGGGAGAGTACGGCGTCGATTCTAAAGTTGGGAGGA190.33153027394870005No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC160.279183388588379No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG150.2617344268016053No Hit
CCATTAGTGAGCTGGCCACCCTGTGATGATTGATGCCGAGTTAGACCAAG140.24428546501483162No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG140.24428546501483162No Hit
CCATTAGTGAGCTGGCCAACCCTTGATGATTGATGCCGAGTTAGACCAAG140.24428546501483162No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG130.22683650322805793No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTATGCCGAGTTAGACCAAG120.20938754144128424No Hit
CCATTGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG110.19193857965451055No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGATAGACCAA100.17448961786773687No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCCCACAGAAGCATATCCAG100.17448961786773687No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG100.17448961786773687No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATGATGCCGAGTTAGACCAAG100.17448961786773687No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCCGAGTTAGACCAAG90.15704065608096318No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG90.15704065608096318No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGCCACACAGAAGCATATCCAG90.15704065608096318No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG90.15704065608096318No Hit
CCATAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG90.15704065608096318No Hit
CCATTAGTGAGTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG80.1395916942941895No Hit
CCATTAGTGGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG80.1395916942941895No Hit
CCATTAGTGAGCTGGTCAACCCTGTGATGATTGATGCCGAGTTAGACCAA80.1395916942941895No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATATTGATGCCGAGTTAGACCAAG80.1395916942941895No Hit
CCATTAGTGAGCTGGCCAACCAAGGTTGCTGGCTATACCTCGAGAAATCG70.12214273250741581No Hit
CCATTAGTGAGCTGGCCAACCCTGTGAGATTGATGCCGAGTTAGACCAAG70.12214273250741581No Hit
CCATTAGTGAGCTGGCCAACCCTGGATGATTGATGCCGAGTTAGACCAAG70.12214273250741581No Hit
CCATTATGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG70.12214273250741581No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCGCACAGAAGCATATCCAG70.12214273250741581No Hit
AGATCATGTACGTCGGGGAGAGTACGGCGTCGATTCTAAAGTTGGTGGGA70.12214273250741581No Hit
CCATTAGTGAGCTGGCAAACCCTGTGATGATTGATGCCGAGTTAGACCAA70.12214273250741581No Hit
CCATTAGGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG70.12214273250741581No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAACATATCCAG60.10469377072064212No Hit
CCATTATGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG60.10469377072064212No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCAAATCCAG60.10469377072064212No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAGGCATATCCAG60.10469377072064212No Hit
CCTTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG60.10469377072064212No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCGAGTTAGACCAAG60.10469377072064212No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATCCGAGTTAGACCAAG60.10469377072064212No Hit
CCATTAGTGAGCTGGCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG60.10469377072064212No Hit
CCATTAGTGAGCTGGCCAACCTGTGATGATTGATGCCGAGTTAGACCAAG60.10469377072064212No Hit
CCATTAGTAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG60.10469377072064212No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACGCAGAAGCATATCCAG60.10469377072064212No Hit
CCATTAGTGAGTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG60.10469377072064212No Hit
AGATCATTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG60.10469377072064212No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)