FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9915
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT500650.48915784165406No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA104510.53958648512355No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT8068.129097327281896No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA1291.3010590015128594No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGGTCTTTGTAGAACCA630.6354009077155824No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG520.524457892082703No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG380.3832576903681291No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT300.30257186081694404No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG300.30257186081694404No Hit
GCATCAATCTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG290.29248613212304586No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG280.28240040342914774No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG270.2723146747352496No Hit
GATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG270.2723146747352496No Hit
GCATAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG270.2723146747352496No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGACGTCCCTCTT270.2723146747352496No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGCTGTCCCTCTT240.2420574886535552No Hit
CATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG240.2420574886535552No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCCCTT230.2319717599596571No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCAT210.2118003025718608No Hit
GCATCAATCATCACAGGGTTGGCTCGAGAAATCGCGAGATGTCCCTCTTG200.2017145738779627No Hit
GCTCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG200.2017145738779627No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGCCCCTCTT200.2017145738779627No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGAAGTCCCTCTT200.2017145738779627No Hit
ACGTTGCCTTTATCCTCCCAACTTTAGAATCGACGCCGTACTCTCCCCGA190.19162884518406456No Hit
GCATCAATCATCACGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG190.19162884518406456No Hit
GCATCAATCACACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG180.1815431164901664No Hit
GCATCAATCATCACCGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT180.1815431164901664No Hit
GCATCAATCATCACAGGGTGGACTCGAGAAATCGCGAGATGTCCCTCTTG170.1714573877962683No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGGTGTCCCTCTT170.1714573877962683No Hit
GCATCAAACATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT170.1714573877962683No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGGGATGTCCCTCTT170.1714573877962683No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCACTT170.1714573877962683No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTA170.1714573877962683No Hit
GCATCAATCATCACAGGGTTGGACTCGGGAAATCGCGAGATGTCCCTCTT150.15128593040847202No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAGTCGCGAGATGTCCCTCTT150.15128593040847202No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC140.14120020171457387No Hit
GCATCAATCATCACAGGGGTGGACTCGAGAAATCGCGAGATGTCCCTCTT130.13111447302067575No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAACTCGCGAGATGTCCCTCTT130.13111447302067575No Hit
GCATCAATCATCACAGGGTTGGACTCGAGGAATCGCGAGATGTCCCTCTT130.13111447302067575No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTC130.13111447302067575No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGTGAGATGTCCCTCTT120.1210287443267776No Hit
GCATCAATCATCCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT120.1210287443267776No Hit
GCATCAATCAACACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT120.1210287443267776No Hit
GCATCAATCATCACAGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG120.1210287443267776No Hit
GCATCAATCATCACAGGGTTGGACACGAGAAATCGCGAGATGTCCCTCTT120.1210287443267776No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTACCTCTT110.11094301563287948No Hit
GCATCAATCATCACAGCGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT110.11094301563287948No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC110.11094301563287948No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGTCTTTGTAGAAC110.11094301563287948No Hit
GCATCAATCATCACAGGGTTGACTCGAGAAATCGCGAGATGTCCCTCTTG110.11094301563287948No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGAGAGATGTCCCTCTT110.11094301563287948No Hit
GCATCAATCATCACAGGGTTGGGCTCGAGAAATCGCGAGATGTCCCTCTT110.11094301563287948No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCATCTT110.11094301563287948No Hit
GCCTCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT100.10085728693898136No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTGCCTCTT100.10085728693898136No Hit
ACATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT100.10085728693898136No Hit
GCATCACTCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT100.10085728693898136No Hit
GCACAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG100.10085728693898136No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)