FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3509
Total Bases505.3 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT179051.01168424052437No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA47113.422627529210601No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA581.6528925619834711No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGGTCTTTGTAGAACCA361.0259333143345684No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT220.6269592476489028No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT190.5414648047876889No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAACTGGT140.39897406668566543No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT130.3704759190652608No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGAGGGGCCCATTCGATCTGGT120.3419777714448561No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG120.3419777714448561No Hit
ACGTTGCCTTTATCCTCCCAACTTTAGAATCGACGCCGTACTCTCCCCGA110.3134796238244514No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGCTCTGGT110.3134796238244514No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCAGGT110.3134796238244514No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG100.28498147620404674No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATATGGT100.28498147620404674No Hit
ACGTTGCCTATATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT90.2564833285836421No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG90.2564833285836421No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGGTCTTTGTAGAACCAT90.2564833285836421No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCGTTCGATCTGGT80.2279851809632374No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCACTCGATCTGGT80.2279851809632374No Hit
ACTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG70.19948703334283271No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTATGGGGTCTTTGTAGAAC70.19948703334283271No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGGGTGGGGCCCATTCGATCTGGT70.19948703334283271No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGACCTGGT70.19948703334283271No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG70.19948703334283271No Hit
ACGTTGCCTTTATCCTCCCAGATCAAGGAGTGGGGCCCATTCGATCTGGT60.17098888572242804No Hit
ACGTTGACTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT60.17098888572242804No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGA60.17098888572242804No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGTCTTTGTAGAAC60.17098888572242804No Hit
ACGTTGCCTTTATCCTCCCAGATCCGGGAGTGGGGCCCATTCGATCTGGT50.14249073810202337No Hit
GCATCAATCATCACAGGGTTTGGCCAGCTCACTAATGGGGTCTTTGTAGA50.14249073810202337No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT50.14249073810202337No Hit
ACGTTGCCTTTATCCTCCCAGAACCAGGAGTGGGGCCCATTCGATCTGGT50.14249073810202337No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC50.14249073810202337No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTAGATCTGGT50.14249073810202337No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATTTGGT50.14249073810202337No Hit
ACGTAGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT50.14249073810202337No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG50.14249073810202337No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCCGGT40.1139925904816187No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTAGT40.1139925904816187No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCAATCTGGT40.1139925904816187No Hit
GCATCAATCATCACAGGGTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC40.1139925904816187No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGAGGCCCATTCGATCTGGT40.1139925904816187No Hit
GCATCAATCATCACAGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC40.1139925904816187No Hit
ACGTTGCCTTTATCCTCCCCGATCCAGGAGTGGGGCCCATTCGATCTGGT40.1139925904816187No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGGCTTTGTAGAA40.1139925904816187No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAATCGATCTGGT40.1139925904816187No Hit
ACGTTGCCTTTATCCTCCCAGCTCCAGGAGTGGGGCCCATTCGATCTGGT40.1139925904816187No Hit
ACGTTGCCTTTATCCTCCCACAGAGCCTTGGCATGCTCATAGAATTCGGG40.1139925904816187No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGGTCTTTGTAGAAC40.1139925904816187No Hit
ACGTTGCCTTTAACCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT40.1139925904816187No Hit
GATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC40.1139925904816187No Hit
ACGTTGCCTTTATCCCCGACGTACATGATCTGGTCTTTGTAGAACCATGT40.1139925904816187No Hit
ACGTTGCCTTTATCCTCCCACCAACTTTAGAATCGACGCCGTACTCTCCC40.1139925904816187No Hit
CATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC40.1139925904816187No Hit
ACGTTGCCTTTATCCTCCCAGACCCAGGAGTGGGGCCCATTCGATCTGGT40.1139925904816187No Hit
ACGTTGCCTTTCTCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT40.1139925904816187No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATACGATCTGGT40.1139925904816187No Hit
ACGATGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT40.1139925904816187No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)