FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12838
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG807262.8758373578439No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAA12699.884717245676896No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC7906.153606480760243No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCCGAGTTAGACCAA1671.3008256737809627No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTCGAGTTAGACCAAGTC1050.8178844056706652No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG720.5608350210313133No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG520.40504751518928184No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG520.40504751518928184No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG460.3583112634366724No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG440.34273251285246925No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG430.33494313756036764No Hit
AGATCTGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG390.3037856363919613No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCCCACAGAAGCATATCCAG380.2959962610998598No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCGCACAGAAGCATATCCAG360.28041751051565666No Hit
AGATATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG350.27262813522355506No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGCCACACAGAAGCATATCCAG310.24147063405514876No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACGGAAGCATATCCAG300.2336812587630472No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACGCAGAAGCATATCCAG300.2336812587630472No Hit
AGATCATGTACGTCGGGGACGTCCGCGGCGTCACACAGAAGCATATCCAG290.22589188347094563No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAGGCATATCCAG260.20252375759464092No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCGTATCCAG260.20252375759464092No Hit
AGTCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG250.19473438230253934No Hit
AGATCATTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG250.19473438230253934No Hit
AGATCATGTACGTCGGGGACGTCCACAGCGTCACACAGAAGCATATCCAG240.18694500701043776No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATACCCAG240.18694500701043776No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG230.1791556317183362No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCGG220.17136625642623463No Hit
AGATCATGACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG220.17136625642623463No Hit
AGATCATGTACGTCGGGGACGCCCGCAGCGTCACACAGAAGCATATCCAG210.16357688113413305No Hit
AGATCATGTACGTGGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG200.15578750584203147No Hit
AGATCATGTACGTCGGGGACGTCCGCAGTGTCACACAGAAGCATATCCAG200.15578750584203147No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC190.1479981305499299No Hit
AGATCATGTACGTCGGGGCCGTCCGCAGCGTCACACAGAAGCATATCCAG190.1479981305499299No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTCGAGTTAGACCAAGTCT190.1479981305499299No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCCGAGTTAGACCAAG180.14020875525782833No Hit
AGATCATGTACGTCGGGGAGAGTACGGCGTCGATTCTAAAGTTGGGAGGA180.14020875525782833No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTATGCCGAGTTAGACCAAG170.13241937996572675No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATGTCCAG170.13241937996572675No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCAGATCCAG170.13241937996572675No Hit
AGATCATGTACGTCGGGGACGTCCGTAGCGTCACACAGAAGCATATCCAG170.13241937996572675No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATACAG170.13241937996572675No Hit
AGATCATGTCGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG150.1168406293815236No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG140.10905125408942204No Hit
GATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG140.10905125408942204No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG140.10905125408942204No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGTATATCCAG140.10905125408942204No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGGAGCATATCCAG130.10126187879732046No Hit
AGATCATGTACGTCGGGGACGTCTGCAGCGTCACACAGAAGCATATCCAG130.10126187879732046No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)