FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7731
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG384749.76070366058725No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAA106613.788643125080844No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC104313.491139567973095No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCCGAGTTAGACCAA1692.1860043978786705No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTCGAGTTAGACCAAGTC831.0735997930410037No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG390.5044625533566163No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG390.5044625533566163No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG330.42685292976329064No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG230.29750355710774806No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC220.2845686198421938No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG220.2845686198421938No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG190.24576380804553097No Hit
AGATATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG180.23282887077997672No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCCCACAGAAGCATATCCAG180.23282887077997672No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCGG180.23282887077997672No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATACCCAG180.23282887077997672No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGCCACACAGAAGCATATCCAG170.21989393351442243No Hit
AGATCTGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG160.20695899624886818No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCCGAGTTAGACCAA160.20695899624886818No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG160.20695899624886818No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAGGCATATCCAG150.19402405898331393No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTCGAGTTAGACCAAGTCT150.19402405898331393No Hit
AGATCATGTACGTCGGGGACGTCCACAGCGTCACACAGAAGCATATCCAG150.19402405898331393No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG140.18108912171775968No Hit
AGATCATGTACGTCGGGGACGTCCGCGGCGTCACACAGAAGCATATCCAG140.18108912171775968No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATGATGCCGAGTTAGACCAAG140.18108912171775968No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACGGAAGCATATCCAG130.1681541844522054No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCGCACAGAAGCATATCCAG130.1681541844522054No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACGCAGAAGCATATCCAG130.1681541844522054No Hit
AGATCATGACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG130.1681541844522054No Hit
AGATCATGTACGTCGGGGACGTCCGCAGTGTCACACAGAAGCATATCCAG130.1681541844522054No Hit
AATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG120.15521924718665114No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG110.1422843099210969No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGGAGCATATCCAG110.1422843099210969No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCAGATCCAG110.1422843099210969No Hit
CCATTAGTGAGCTGGCCAACCAAGGTTGCTGGCTATACCTCGAGAAATCG100.1293493726555426No Hit
CCATTAGTGAGCTGGCCAACCCTGGATGATTGATGCCGAGTTAGACCAAG100.1293493726555426No Hit
GATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG90.11641443538998836No Hit
AGATCATGTACGTCGGGGACGCCCGCAGCGTCACACAGAAGCATATCCAG90.11641443538998836No Hit
AGATCATGTACGTGGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG80.10347949812443409No Hit
AGATCATGTACGTCGGGGACATCCGCAGCGTCACACAGAAGCATATCCAG80.10347949812443409No Hit
AGTCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG80.10347949812443409No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGACACACAGAAGCATATCCAG80.10347949812443409No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCACATCCAG80.10347949812443409No Hit
CCATTAGTAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG80.10347949812443409No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCGTATCCAG80.10347949812443409No Hit
CCATTAGTGAGCTGACCAACCCTGTGATGATTGATGCCGAGTTAGACCAA80.10347949812443409No Hit
AGATCATTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG80.10347949812443409No Hit
AGATCATGTACGTCGGGGAGAGTACGGCGTCGATTCTAAAGTTGGGAGGA80.10347949812443409No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)