FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7501
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length134-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT263835.1686441807759No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT125816.77109718704173No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA94012.531662445007333No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA1501.9997333688841485No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGGTCTTTGTAGAACCA1011.34648713504866No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG280.3732835621917078No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGCTGTCCCTCTT190.25329956005865883No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCCCTT190.25329956005865883No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT180.23996800426609785No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGTCTTTGTAGAAC180.23996800426609785No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT170.22663644847353684No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGGTCTTTGTAGAAC170.22663644847353684No Hit
GCATAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG160.21330489268097585No Hit
GCATCAATCTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG150.1999733368884149No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG150.1999733368884149No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG150.1999733368884149No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG140.1866417810958539No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGAAGTCCCTCTT140.1866417810958539No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGCCCCTCTT130.17331022530329288No Hit
ACGTTGCCTTTATCCTCCCAACTTTAGAATCGACGCCGTACTCTCCCCGA130.17331022530329288No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC130.17331022530329288No Hit
GATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG130.17331022530329288No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGGTGTCCCTCTT120.15997866951073192No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAACTCGCGAGATGTCCCTCTT120.15997866951073192No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC120.15997866951073192No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAGTCGCGAGATGTCCCTCTT110.1466471137181709No Hit
GCTCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC110.1466471137181709No Hit
GCATCAAACATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT100.13331555792560992No Hit
GCATCAATCATCACGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG100.13331555792560992No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGGTCTTTGTAGAACCAT100.13331555792560992No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG100.13331555792560992No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT90.11998400213304893No Hit
GCATCAATCATCCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT90.11998400213304893No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTC90.11998400213304893No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTA90.11998400213304893No Hit
GCATCAATCATCACAGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG90.11998400213304893No Hit
GCATCACTCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT90.11998400213304893No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC90.11998400213304893No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCAT80.10665244634048793No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG80.10665244634048793No Hit
GCATCAATCAACACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT80.10665244634048793No Hit
GCATCAATCATCACCGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT80.10665244634048793No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATATGGT80.10665244634048793No Hit

[FAIL]Adapter Content

Adapter graph