FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5748
Total Bases832.3 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG173230.132219902574807No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAA124821.711899791231733No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC119520.789839944328463No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTCGAGTTAGACCAAGTC1883.2707028531663185No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCCGAGTTAGACCAA1342.3312456506610997No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC330.5741127348643006No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG260.4523312456506611No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTCGAGTTAGACCAAGTCT220.3827418232428671No Hit
AGATCATGTACGTCGGGGAGAGTACGGCGTCGATTCTAAAGTTGGGAGGA210.36534446764091855No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG180.31315240083507306No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCCGAGTTAGACCAA160.2783576896311761No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATGATGCCGAGTTAGACCAAG150.2609603340292276No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG130.22616562282533054No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG120.20876826722338201No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCCGAGTTAGACCAAG120.20876826722338201No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG120.20876826722338201No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG120.20876826722338201No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAGGCATATCCAG100.17397355601948505No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG100.17397355601948505No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCGCACAGAAGCATATCCAG100.17397355601948505No Hit
CCATTAGTGAGTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG100.17397355601948505No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGATATC90.15657620041753653No Hit
CCATTAGTGAGCTGGCCAACCCTGGATGATTGATGCCGAGTTAGACCAAG90.15657620041753653No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG90.15657620041753653No Hit
CCATTATGAGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG80.13917884481558804No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTATGCCGAGTTAGACCAAG80.13917884481558804No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACGGAAGCATATCCAG80.13917884481558804No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATCCGAGTTAGACCAAG80.13917884481558804No Hit
CCATTAGTGGCTGGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG80.13917884481558804No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACGCAGAAGCATATCCAG80.13917884481558804No Hit
CCATTAGTGAGCTGCCAACCCTGTGATGATTGATGCCGAGTTAGACCAAG80.13917884481558804No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGTGCCGAGTTAGACCAAG80.13917884481558804No Hit
AGATCATTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG80.13917884481558804No Hit
CCATTAGTGGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG70.12178148921363954No Hit
CCATTAGTGAGCTGGCCAACCCTGTGAGATTGATGCCGAGTTAGACCAAG70.12178148921363954No Hit
AGATCATGTACGTGGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG70.12178148921363954No Hit
CCATTAGTGAGCTGGCCAACCTGTGATGATTGATGCCGAGTTAGACCAAG70.12178148921363954No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCGAGATAGACCAA60.10438413361169101No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCCCACAGAAGCATATCCAG60.10438413361169101No Hit
CCTTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG60.10438413361169101No Hit
CCATTAGTGAGCTGGCCAAACCCTGTGATGATTGATGCCGAGTTAGACCA60.10438413361169101No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGGAGCATATCCAG60.10438413361169101No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCAGATCCAG60.10438413361169101No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCGG60.10438413361169101No Hit
CCATTAGTAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG60.10438413361169101No Hit
CCATTAGTGAGCTGGCCAACCCTTGATGATTGATGCCGAGTTAGACCAAG60.10438413361169101No Hit
CCATAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG60.10438413361169101No Hit
CCATTAGAGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC60.10438413361169101No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATATTGATGCCGAGTTAGACCAAG60.10438413361169101No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATACCCAG60.10438413361169101No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)