FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2867
Total Bases413.1 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT131145.72724101848622No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA51117.823508894314617No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGGTCTTTGTAGAACCA602.0927799093128705No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGGTCTTTGTAGAA521.8137425880711544No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGTCCATTCGATCTGGT250.8719916288803629No Hit
ACGTTGCCTTTATCCTCCCAACTTTAGAATCGACGCCGTACTCTCCCCGA180.6278339727938612No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG140.48831531217300317No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT120.4185559818625741No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG110.38367631670735963No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCAGGT110.38367631670735963No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT100.3487966515521451No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG90.3139169863969306No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAACTGGT80.2790373212417161No Hit
GCATCAATCATCACAGGGTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC70.24415765608650158No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGGTCTTTGTAGAAC70.24415765608650158No Hit
ACGTTGCCTTTAACCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT70.24415765608650158No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGGTCTTTGTAGAACCAT70.24415765608650158No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGCTCTGGT70.24415765608650158No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG70.24415765608650158No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATATGGT70.24415765608650158No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC70.24415765608650158No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG70.24415765608650158No Hit
ACGGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT60.20927799093128704No Hit
ACGTTGCCTTTATCCTCCCACCAACTTTAGAATCGACGCCGTACTCTCCC60.20927799093128704No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC60.20927799093128704No Hit
ACGATGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT60.20927799093128704No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGTGCCCATTCGATCTGGT50.17439832577607256No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC50.17439832577607256No Hit
GCATCAATCTCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC50.17439832577607256No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTATGGGGTCTTTGTAGAAC50.17439832577607256No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAATCGATCTGGT50.17439832577607256No Hit
GCATCAATCATCACAGGGTTGGCCAGCTAACTAATGGGGTCTTTGTAGAA50.17439832577607256No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGACCCATTCGATCTGGT50.17439832577607256No Hit
ACGTTGCCTTTATCCTCCCAGACCCAGGAGTGGGGCCCATTCGATCTGGT50.17439832577607256No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATACGATCTGGT50.17439832577607256No Hit
ACGTTGCCTTTATCCTCCCAGATCCCGGAGTGGGGCCCATTCGATCTGGT40.13951866062085805No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC40.13951866062085805No Hit
ACTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG40.13951866062085805No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATCCGATCTGGT40.13951866062085805No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGCGGGGCCCATTCGATCTGGT40.13951866062085805No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGAGGCCCATTCGATCTGGT40.13951866062085805No Hit
GCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT40.13951866062085805No Hit
ACGTTGACTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT40.13951866062085805No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCGGGT40.13951866062085805No Hit
ACGTTGCCTATATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT40.13951866062085805No Hit
ACGTTGCCTTTATCCTCCCGGATCCAGGAGTGGGGCCCATTCGATCTGGT40.13951866062085805No Hit
GCATCAATCATCACGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC40.13951866062085805No Hit
ACGTTGCCTTTATCCTCCCAGATCCTGGAGTGGGGCCCATTCGATCTGGT40.13951866062085805No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT40.13951866062085805No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG40.13951866062085805No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGC40.13951866062085805No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGAGGGGCCCATTCGATCTGGT40.13951866062085805No Hit
GCATCAATCATCACAGGGTTGGCAGCTCACTAATGGGGTCTTTGTAGAAC40.13951866062085805No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGACCTGGT40.13951866062085805No Hit
ACGTAGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT40.13951866062085805No Hit
ACGTTCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG40.13951866062085805No Hit
ACGTTGCCTTTTCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG40.13951866062085805No Hit
GCATCAATCATCACAGGGTTGGTCAGCTCACTAATGGGGTCTTTGTAGAA40.13951866062085805No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC40.13951866062085805No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCCGGT30.10463899546564352No Hit
GCATCAATCATCACAGGGTTGGCCAACTCACTAATGGGGTCTTTGTAGAA30.10463899546564352No Hit
GCATCAATCATCACAGGGTTGGCCACTCACTAATGGGGTCTTTGTAGAAC30.10463899546564352No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGGGTGGGGCCCATTCGATCTGGT30.10463899546564352No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACAATGGGGTCTTTGTAGAAC30.10463899546564352No Hit
GCATAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC30.10463899546564352No Hit
ACGTTGCCTTTATCCTCCCGATCCAGGAGTGGGGCCCATTCGATCTGGTG30.10463899546564352No Hit
GATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC30.10463899546564352No Hit
ACGTTGCCTTTATCATCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT30.10463899546564352No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCTCATTCGATCTGGT30.10463899546564352No Hit
ACGTTGCCTTTATCCTCCCAGAACCAGGAGTGGGGCCCATTCGATCTGGT30.10463899546564352No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGACCATTCGATCTGGT30.10463899546564352No Hit
GCTCAATCATCACAGGGTTGGCCAGCTCACTAATGGGGTCTTTGTAGAAC30.10463899546564352No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGG30.10463899546564352No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGTCTTTGTAGAAC30.10463899546564352No Hit
GCATCAATCATCACAGGGTTGGCCAGCCACTAATGGGGTCTTTGTAGAAC30.10463899546564352No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTAGATCTGGT30.10463899546564352No Hit
GCATCAATCATCACAGGGTTGGCCAGTCACTAATGGGGTCTTTGTAGAAC30.10463899546564352No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTCGT30.10463899546564352No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)