FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008132255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008132255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7922
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG400050.49229992426155No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG205925.990911386013632No Hit
AAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT790.9972229235041655No Hit
CAAGTGTAAGCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT710.8962383236556425No Hit
TGTCCCTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG600.7573844988639232No Hit
CAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT420.5301691492047462No Hit
CAAGTGTAAGCCTGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT310.39131532441302697No Hit
CAAGTGTAAGCCCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT260.3281999495077001No Hit
CAAGTGTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT250.3155768745266347No Hit
TGTACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC230.2903307245645039No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGCCCCCCCAGGTGTGTG200.25246149962130776No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCAGGTGTGTGT200.25246149962130776No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGAGTG160.20196919969704621No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGCGTG150.1893461247159808No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGCGTGTG140.17672304973491543No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTCGTCCCCCCAGGTGTGTG140.17672304973491543No Hit
CAAGTGTAAGCCTCGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT130.16409997475385005No Hit
TGTCACTTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC130.16409997475385005No Hit
CAAGTTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT120.15147689977278464No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGAGTGTG120.15147689977278464No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGCG120.15147689977278464No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGGGTGTG110.13885382479171926No Hit
TGTCACTCTCATCGCTGTCGTGGCACCCCGGGAACAGCTTCCCCGCGCGG110.13885382479171926No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGAAACAGCTTCCCCGCGCGG110.13885382479171926No Hit
TGTCACTCTCATCCCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG110.13885382479171926No Hit
TGTCATCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC110.13885382479171926No Hit
TGTCACTCTCACGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC100.12623074981065388No Hit
CAAGTGTAAGCCTCGGCAAACAGGGCCTGGCTTGTCCCCCCAGGTGTGTG100.12623074981065388No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTAGTCCCCCCAGGTGTGTG100.12623074981065388No Hit
TTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC100.12623074981065388No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCTCCCCAGGTGTGTG100.12623074981065388No Hit
CAATGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT100.12623074981065388No Hit
CAAGGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT90.11360767482958849No Hit
TGTCACTCTCATCGCTGTCGTGGCGCACCGGGAACAGCTTCCCCGCGCGG90.11360767482958849No Hit
CAAGTGTAAGCCTCGGCAAACGAGGCCTGGCTTGTCCCCCCAGGTGTGTG90.11360767482958849No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTTCCCCCAGGTGTGTG90.11360767482958849No Hit
CAAGTGTAAGCCTCGGCAAGCAAGGCCTGGCTTGTCCCCCCAGGTGTGTG90.11360767482958849No Hit
CAAGTGTAACCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT80.10098459984852311No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCCGGCTTGTCCCCCCAGGTGTGTG80.10098459984852311No Hit
CAAGTGTAAGCCTGGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG80.10098459984852311No Hit
TGTCACTCTCTCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC80.10098459984852311No Hit
CAAGTGTAAGCGTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG80.10098459984852311No Hit
CAAGTGTAAGCCTCGGCAAACAAGGTCTGGCTTGTCCCCCCAGGTGTGTG80.10098459984852311No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCCTCCCCGCGCGG80.10098459984852311No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)