FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008132413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008132413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9465
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG552558.3729529846804No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG156916.57686212361331No Hit
CAAGTGTAAGCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT1061.1199154780771263No Hit
AAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT1041.09878499735869No Hit
CAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT550.5810882197569995No Hit
CAAGTGTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT490.5176967776016904No Hit
TGTCCCTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG400.4226096143687269No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCAGGTGTGTGT390.4120443740095087No Hit
CAAGTGTAAGCCCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT370.3909138932910724No Hit
CAAGTGTAAGCCTGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT370.3909138932910724No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGCCCCCCCAGGTGTGTG300.31695721077654515No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCCTGTCCCCCCAGGTGTGTG220.2324352879027998No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTCGTCCCCCCAGGTGTGTG200.21130480718436345No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGCGTGTG190.20073956682514527No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGCG190.20073956682514527No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGAGTGTG180.1901743264659271No Hit
CAAGTGTAAGCCTCGGCAAACGAGGCCTGGCTTGTCCCCCCAGGTGTGTG170.17960908610670895No Hit
TGTCACTCTCATCCCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG170.17960908610670895No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCCGGCTTGTCCCCCCAGGTGTGTG160.16904384574749076No Hit
CAAGTTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT160.16904384574749076No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGAG160.16904384574749076No Hit
CAAGTGTAAGCCTCGGCAAGCAAGGCCTGGCTTGTCCCCCCAGGTGTGTG160.16904384574749076No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCGGGTGTGTG150.15847860538827258No Hit
CAAGTGTAAGCCTGGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG150.15847860538827258No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGTTTGTCCCCCCAGGTGTGTG150.15847860538827258No Hit
CAAGTGTAAGCCACGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG150.15847860538827258No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGAGTG140.14791336502905442No Hit
CAATGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT140.14791336502905442No Hit
CAAGGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT130.13734812466983626No Hit
CAAGTGTAAGCCGCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG130.13734812466983626No Hit
TGTCACTTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC130.13734812466983626No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGCGTG130.13734812466983626No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGGGTGTG120.12678288431061807No Hit
TGTCACTCTATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC120.12678288431061807No Hit
CAAGTGTAAGCCTCGGCAAACAGGGCCTGGCTTGTCCCCCCAGGTGTGTG110.1162176439513999No Hit
TGTACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC110.1162176439513999No Hit
CAAGTGTAAGCCTCGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT100.10565240359218173No Hit
CAAGTGTAAGCCTCGGTAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG100.10565240359218173No Hit
TGTCACTCTCATCGCTGTCGTGGCACCCCGGGAACAGCTTCCCCGCGCGG100.10565240359218173No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)