FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008132439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008132439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12939
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG838864.8272664038952No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG155011.979287425612489No Hit
CAAGTGTAAGCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT1401.0820001545714506No Hit
AAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT1301.0047144292449184No Hit
CAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT770.5951000850142978No Hit
CAAGTGTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT700.5410000772857253No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCAGGTGTGTGT600.4637143519591932No Hit
CAAGTGTAAGCCTGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT420.3246000463714352No Hit
CAAGTGTAAGCCCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT410.31687147383878195No Hit
TGTCCCTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG360.27822861117551584No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGCGTG350.27050003864286265No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGAGTGTG300.2318571759795966No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGCCCCCCCAGGTGTGTG290.22412860344694338No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGCG290.22412860344694338No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGGGTGTG270.20867145838163692No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGCGTGTG260.2009428858489837No Hit
CAAGTTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT250.19321431331633046No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGAGTG250.19321431331633046No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTCGTCCCCCCAGGTGTGTG240.18548574078367724No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCCGGCTTGTCCCCCCAGGTGTGTG230.17775716825102403No Hit
CAAGTGTAAGCCTCGGCAAACAGGGCCTGGCTTGTCCCCCCAGGTGTGTG220.1700285957183708No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCCTGTCCCCCCAGGTGTGTG210.1623000231857176No Hit
CAAGTGTAAGCCTCGGCAAGCAAGGCCTGGCTTGTCCCCCCAGGTGTGTG210.1623000231857176No Hit
CAAGTGAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT190.14684287812041116No Hit
CAATGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT180.13911430558775792No Hit
CAAGTGTAACCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT170.1313857330551047No Hit
CAAGTGTAAGCCTGGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG170.1313857330551047No Hit
CAAGTGTAAGCCTCGGCAAACAAGACCTGGCTTGTCCCCCCAGGTGTGTG160.12365716052245149No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCGGGTGTGTG160.12365716052245149No Hit
TGTCACTCTCATCGCTGTCGTGGCACCCCGGGAACAGCTTCCCCGCGCGG150.1159285879897983No Hit
CAAGTGTAAGCCGCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG150.1159285879897983No Hit
CAAGTGTAAGCCTCGGCAAACAATGCCTGGCTTGTCCCCCCAGGTGTGTG140.10820001545714508No Hit
CAAGGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT130.10047144292449185No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTATCCCCCCAGGTGTGTG130.10047144292449185No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTATGTG130.10047144292449185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)