FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008133183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008133183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14474
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG764552.81884758877988No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG346323.925659803786097No Hit
CAAGTGTAAGCCTCGGCAAGCAAGGCCTGGCTTGTCCCCCCAGGTGTGTG1651.1399751278153931No Hit
CAAGTGTAAGCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT880.6079867348348763No Hit
TGTCCCTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG830.5734420339919857No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTA800.5527152134862512No Hit
CAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT600.4145364101146884No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCAGGTGTGTGT560.38690064944037583No Hit
AAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT500.34544700842890697No Hit
CAAGTGTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT470.3247201879231726No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGCCCCCCCAGGTGTGTG420.2901754870802819No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGCGTG360.24872184606881304No Hit
CAAGTGTAAGCCTCTGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG290.2003592648887661No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGCGTGTG290.2003592648887661No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTCGTCCCCCCAGGTGTGTG270.1865413845516098No Hit
CAAGTGTAAGCCTCGGCAAACAAGACCTGGCTTGTCCCCCCAGGTGTGTG260.17963244438303164No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCGGGTGTGTG260.17963244438303164No Hit
TGTACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC230.15890562387729723No Hit
TGTCCTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC230.15890562387729723No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCCGGCTTGTCCCCCCAGGTGTGTG220.15199668370871908No Hit
TGTCACTCTATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC220.15199668370871908No Hit
TGTCACTTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC220.15199668370871908No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGCG220.15199668370871908No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCCTGTCCCCCCAGGTGTGTG210.14508774354014095No Hit
CAATGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT200.1381788033715628No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGAGTG190.13126986320298467No Hit
TGTCACTCTCATCCCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG190.13126986320298467No Hit
TGTCATCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC190.13126986320298467No Hit
CAAGTGTAAGCCTCGGCAAACAGGGCCTGGCTTGTCCCCCCAGGTGTGTG180.12436092303440652No Hit
TGTCACTCTCATCGCTGTCGTGGCACCCCGGGAACAGCTTCCCCGCGCGG170.11745198286582839No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGAG170.11745198286582839No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACGGCTTCCCCGCGCGG170.11745198286582839No Hit
TGTCACTCTCACGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC160.11054304269725024No Hit
TGTCACCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC160.11054304269725024No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGACTTGTCCCCCCAGGTGTGTG150.1036341025286721No Hit
CAAGTGTAAGCCTCGGCAAACAAGGTCTGGCTTGTCCCCCCAGGTGTGTG150.1036341025286721No Hit
CAAGTTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT150.1036341025286721No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGAGTGTG150.1036341025286721No Hit
CAAGTGTAAGCCTCGGAAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG150.1036341025286721No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)