FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008133342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008133342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10808
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG710665.74759437453737No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA210219.44855662472243No Hit
CTTCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA770.7124352331606217No Hit
CTTCCAGGCCTCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA630.582901554404145No Hit
CTTCCAGGCCTCCTAGTGTTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG590.5458919319022946No Hit
CTTCCAGGCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA550.5088823094004441No Hit
TTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA400.3700962250185048No Hit
CATCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG300.27757216876387864No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCCCCTCCTCCGAGCTCCCAGCAGGG250.23131014063656552No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAACTCCCAGCAGGG240.22205773501110287No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCCCCTCCGAGCTCCCAGCAGGG240.22205773501110287No Hit
TGGGTCATGTGGTTGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG230.21280532938564029No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCCCCCAGCAGGG230.21280532938564029No Hit
CTTCCGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA230.21280532938564029No Hit
CTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA220.20355292376017764No Hit
TGGGTCATGTGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG220.20355292376017764No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCCCCGAGCTCCCAGCAGGG210.19430051813471502No Hit
TGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG190.1757957068837898No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGGGCTCCCAGCAGGG180.16654330125832717No Hit
CTTCCAGGCCTCCTAGTGCTCTAGACTCCTCCTCCGAGCTCCCAGCAGGG180.16654330125832717No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCGGGG170.15729089563286455No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGAGGTGTGTGTTGAGAA170.15729089563286455No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAG150.13878608438193932No Hit
CTTCCAGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA150.13878608438193932No Hit
TGGGTCTGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG140.1295336787564767No Hit
TGGGTATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG140.1295336787564767No Hit
CTTCCAGGCCTCCTAGTGCTCCAGGCTCCTCCTCCGAGCTCCCAGCAGGG130.12028127313101407No Hit
TGGGTCATGTGGTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG130.12028127313101407No Hit
CTTCCAGGCCTCCTGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA120.11102886750555144No Hit
CTTCCAGGCCTCCTAGCGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG120.11102886750555144No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATCCTCAGTGGTGTGTGTTGAGAA120.11102886750555144No Hit
TGGGTCATGTGGTTCGGAGGCTTACACTTGCAAGGAAGACCAAGTCTCTG120.11102886750555144No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGCGTGTGTTGAGAA110.10177646188008882No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)