FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008133459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008133459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10380
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG351633.872832369942195No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG335632.331406551059736No Hit
CAAGTGTAAGCCTCGGCAAGCAAGGCCTGGCTTGTCCCCCCAGGTGTGTG900.8670520231213872No Hit
TGTCCCTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG700.674373795761079No Hit
TGTCACTCTCATCGCTGTCGTGGCACCCCGGGAACAGCTTCCCCGCGCGG680.6551059730250481No Hit
CAAGTGTAAGCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT370.35645472061657035No Hit
TGTCACTCTCATCCCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG360.3468208092485549No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGAGTGTG330.3179190751445087No Hit
TGTCACTCTCATCGCTGTCGTGGCCCACCGGGAACAGCTTCCCCGCGCGG320.3082851637764933No Hit
CAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT290.27938342967244706No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCAGGTGTGTGT270.26011560693641617No Hit
CAAGTGTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT270.26011560693641617No Hit
TGTACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC240.23121387283236997No Hit
TGTCACTCTCCTCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG220.2119460500963391No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGCCCCCCCAGGTGTGTG220.2119460500963391No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGAG210.20231213872832368No Hit
TGTCCTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC210.20231213872832368No Hit
TGTCACTCTCACGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC190.18304431599229287No Hit
TGTCATCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC190.18304431599229287No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGTTTCCCCGCGCGG180.17341040462427745No Hit
TGTCACTCTCAACGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG180.17341040462427745No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGAGTG180.17341040462427745No Hit
TGTCACGCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG180.17341040462427745No Hit
AAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT180.17341040462427745No Hit
CAAGTGTAAGCCTCTGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG170.16377649325626203No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACTGCTTCCCCGCGCGG170.16377649325626203No Hit
TGTCACTCTCATCGCTGTCGCGGCACACCGGGAACAGCTTCCCCGCGCGG150.1445086705202312No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCGGGTGTGTG150.1445086705202312No Hit
TGTCACTCTCTCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC150.1445086705202312No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCTCGCGG150.1445086705202312No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGCGTGTG140.1348747591522158No Hit
TGTCACTTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC140.1348747591522158No Hit
TGTCACTCTCATCGCTGTCGTGGTACACCGGGAACAGCTTCCCCGCGCGG130.1252408477842004No Hit
TGGCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG130.1252408477842004No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCATCCCCGCGCGG130.1252408477842004No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTATCCCCCCAGGTGTGTG130.1252408477842004No Hit
TGTCACTCTATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC130.1252408477842004No Hit
CAAGTGTACGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG130.1252408477842004No Hit
TGTCACTCTCATCGCTGTCGTGGCACGCCGGGAACAGCTTCCCCGCGCGG130.1252408477842004No Hit
CAAGTGTAAGCCTCGGCAAACAGGGCCTGGCTTGTCCCCCCAGGTGTGTG120.11560693641618498No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCACGG120.11560693641618498No Hit
TTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC120.11560693641618498No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGCG120.11560693641618498No Hit
TGTCACTCTCATCGCAGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG120.11560693641618498No Hit
CAAGTGTAAGCCACGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG120.11560693641618498No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGACCCCCCAGGTGTGTG110.10597302504816955No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGGACAGCTTCCCCGCGCGG110.10597302504816955No Hit
CAATGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT110.10597302504816955No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTCGTCCCCCCAGGTGTGTG110.10597302504816955No Hit
CAAGAGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG110.10597302504816955No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGCACAGCTTCCCCGCGCGG110.10597302504816955No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGT110.10597302504816955No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCCTCCCCGCGCGG110.10597302504816955No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)