FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008133525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008133525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9604
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC533355.528946272386506No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT298531.080799666805497No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT660.6872136609745939No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC280.2915451895043732No Hit
TGCTGATCATTGTTGCTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC210.21865889212827988No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC200.20824656393169513No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTACTTCCTC180.1874219075385256No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT180.1874219075385256No Hit
TGCTGACATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT170.17700957934194084No Hit
TGCTGATCATTGTTGCTTTGGGGGCGAGGAAAAGTCTGGAGTATTTCCTC170.17700957934194084No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGGGGAAAAGTCTGGAGTATTTCCT170.17700957934194084No Hit
TGCTGATCATTGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT160.16659725114535612No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT160.16659725114535612No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT160.16659725114535612No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT160.16659725114535612No Hit
TGCGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT150.15618492294877134No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTCCCTC150.15618492294877134No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC150.15618492294877134No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC140.1457725947521866No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGCATTTCCTC140.1457725947521866No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAGGTCTGGAGTATTTCCT130.13536026655560182No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCCGGAGTATTTCCTC120.12494793835901709No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCC120.12494793835901709No Hit
TGCTATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT120.12494793835901709No Hit
TGTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC120.12494793835901709No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT120.12494793835901709No Hit
TCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT110.11453561016243233No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGCCTGGAGTATTTCCTC110.11453561016243233No Hit
TGCTGATATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC110.11453561016243233No Hit
GCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT110.11453561016243233No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAGAAGTCTGGAGTATTTCCT100.10412328196584757No Hit
TGCTGATCATTGTTGCTTTGGGGGGGTGAGGAAAAGTCTGGAGTATTTCC100.10412328196584757No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTGTTTCCTC100.10412328196584757No Hit
TGCTGATCATTGTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC100.10412328196584757No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTACTTCCT100.10412328196584757No Hit
TGCGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC100.10412328196584757No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT100.10412328196584757No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)