FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008140547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008140547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11693
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC1006786.0942444197383No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT1100.940733772342427No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC420.3591892585307449No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC400.3420850081245189No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTCCCTC380.324980757718293No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC380.324980757718293No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT370.31642863251518No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT360.30787650731206706No Hit
TGCTGATCATTGTTGCTTCAAGTGCTTCACAGTGTAGAACCATGTCGTCA350.29932438210895407No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC350.29932438210895407No Hit
TGCTATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT340.2907722569058411No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGGGTATTTCCTC330.28222013170272814No Hit
TGCTGATCATTGTTGCTTTGGGGGCGAGGAAAAGTCTGGAGTATTTCCTC330.28222013170272814No Hit
TGCGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT300.2565637560933892No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCCGGAGTATTTCCTC290.24801163089027625No Hit
GCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT290.24801163089027625No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT270.2309073804840503No Hit
TCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT270.2309073804840503No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGCCTGGAGTATTTCCTC270.2309073804840503No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCCC260.2223552552809373No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC260.2223552552809373No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTACTTCCTC260.2223552552809373No Hit
TGCTGATCATTGTTGATTCAAGTGCTTCACAGTGTAGAACCATGTCGTCA240.20525100487471135No Hit
TGCTGATCATTGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT230.19669887967159838No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT230.19669887967159838No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGCATTTCCTC210.17959462926537245No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT210.17959462926537245No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT200.17104250406225946No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTGTTTCCTC190.1624903788591465No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGGAAAGTCTGGAGTATTTCCTC180.15393825365603353No Hit
TGCTGACATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT160.13683400324980757No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT160.13683400324980757No Hit
TGCTGATCATTTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT130.11117762764046865No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGAAAAAGTCTGGAGTATTTCCTC120.10262550243735567No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)