FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008140694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008140694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8763
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC765787.37875156909735No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC550.6276389364372932No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC550.6276389364372932No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC320.36517174483624326No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCCAAACTCACAC290.3309368937578455No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC290.3309368937578455No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC290.3309368937578455No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC280.3195252767317129No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC270.3081136597055803No Hit
AGCACTTGAATCAACAGGCTTCGGAGACTGAGCCATTTTCAAACTCACAC250.2852904256533151No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCGCAC240.27387880862718245No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC240.27387880862718245No Hit
AGCACTTGAATCAACAGGCTTCAGGGACTGAGCCATTTTCAAACTCACAC230.26246719160104987No Hit
AGCACTTGAATCAACAGGCTTCAGAGGCTGAGCCATTTTCAAACTCACAC230.26246719160104987No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC210.23964395754878468No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACGC190.21682072349651946No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC180.20540910647038688No Hit
AGCACTTGAATCAACAGGCCTCAGAGACTGAGCCATTTTCAAACTCACAC180.20540910647038688No Hit
AGCACTTGAATCAACAGGCTTCAGAGACCGAGCCATTTTCAAACTCACAC170.19399748944425424No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC170.19399748944425424No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAGACTCACAC170.19399748944425424No Hit
AGCACTTGAATCAACAATGATCAGCACCATGTAGACCAAGTCTCTGCTAC160.18258587241812163No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAT160.18258587241812163No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC160.18258587241812163No Hit
ACACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC160.18258587241812163No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCGTTTTCAAACTCACAC150.17117425539198905No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCACTTTCAAACTCACAC150.17117425539198905No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCGAACTCACAC150.17117425539198905No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATCTTCAAACTCACAC140.15976263836585644No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAACCATTTTCAAACTCACAC140.15976263836585644No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGTCATTTTCAAACTCACAC130.14835102133972383No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTCTCAAACTCACAC120.13693940431359122No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAGCTCACAC120.13693940431359122No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTTACAC110.12552778728745864No Hit
AGCACTTGAACAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC110.12552778728745864No Hit
AGCACTTGAATCAACAGGCTGCAGAGACTGAGCCATTTTCAAACTCACAC90.10270455323519344No Hit
AGCACTTGAATCAATAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC90.10270455323519344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)