FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008140731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008140731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8824
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT746984.6441523118767No Hit
TGCTGATCATTGTTGATTCAAGTGCTTCACAGTGTAGAACCATGTCGTCA1361.5412511332728922No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC830.9406165004533092No Hit
TGCTGATCATTGTTGCTTCAAGTGCTTCACAGTGTAGAACCATGTCGTCA760.8612873980054397No Hit
TGCTATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC330.3739800543970988No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAGGTCTGGAGTATTTCCT300.3399818676337262No Hit
TGCTGATATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC270.3059836808703536No Hit
TGCTGATCATTGTTGCTTTGGGGGGGTGAGGAAAAGTCTGGAGTATTTCC270.3059836808703536No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGGGGAAAAGTCTGGAGTATTTCCT260.29465095194922936No Hit
TGTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC260.29465095194922936No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAGAAGTCTGGAGTATTTCCT240.271985494106981No Hit
TGCTGTCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC220.24932003626473254No Hit
GCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC210.23798730734360835No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGGGTATTTCCT190.21532184950135994No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGCCTGGAGTATTTCCT180.20398912058023572No Hit
TGCTGATCATTGTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC180.20398912058023572No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCC180.20398912058023572No Hit
TGCGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC180.20398912058023572No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTCCCT180.20398912058023572No Hit
TGCTGATCATTGTTGCTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC180.20398912058023572No Hit
TGCTGATCATGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC170.19265639165911153No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAGAGTCTGGAGTATTTCCT160.1813236627379873No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATCTCCT160.1813236627379873No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTACTTCCT160.1813236627379873No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCCGGAGTATTTCCT160.1813236627379873No Hit
TGCTGATCTTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC140.1586582048957389No Hit
TGCTGATCATTGTTGCTTTGGGGGGCGAGGAAAAGTCTGGAGTATTTCCT130.14732547597461468No Hit
TCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC130.14732547597461468No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAATATTTCCT110.12466001813236627No Hit
TGCTGATCATTTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC110.12466001813236627No Hit
TGCTGATCATTGTTGCTTGGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT110.12466001813236627No Hit
TGCTGATCATTGTTGTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC100.11332728921124206No Hit
TGCTGACATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC100.11332728921124206No Hit
TGCTGATCATTGTTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCC90.10199456029011786No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGGAAAGTCTGGAGTATTTCCT90.10199456029011786No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)