FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008140825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008140825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18951
Total Bases2.7 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC760540.129808453379766No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT431922.790354071025277No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC358618.922484301619967No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG9625.076249274444621No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT1080.5698907709355706No Hit
AGACCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT510.2691150862751306No Hit
TGGCTCATCTTCAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT450.23745448788982113No Hit
TGGCTCATCTTAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT430.22690095509471797No Hit
TGGCTATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT410.2163474222996148No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG400.21107065590206323No Hit
TGGCTCATCTTCAAACCGTGGGCAACTCTGGCCGGCCTCTGCTGAATGAC380.20051712310696004No Hit
TGGCTCTCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT370.19524035670940848No Hit
AGACCCCTGGAACTGCTACGTGTGCGGGCACAAGGGTACCTACGGGCTGC310.163579758324099No Hit
AGACCCCTGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT300.15830299192654743No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG280.14774945913144424No Hit
AGACCCCTGGAACTGTTACATGTGCGGGCACAAGGGTACCTACGGGCTGC260.1371959263363411No Hit
AGCCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT250.13191915993878953No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTATCTACGGGCTGC250.13191915993878953No Hit
GGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT240.12664239354123794No Hit
GACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT230.12136562714368634No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGT220.11608886074613477No Hit
AGACCCCTGGAACTGCTACATGTGCGGACACAAGGGTACCTACGGGCTGC220.11608886074613477No Hit
TGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT210.1108120943485832No Hit
AGACCCCTGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT210.1108120943485832No Hit
AGACCCCGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT200.10553532795103161No Hit
AACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT200.10553532795103161No Hit
AGACCCCTGGACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT200.10553532795103161No Hit
TGGCTCATTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT190.10025856155348002No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)