FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008140837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008140837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7750
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT590976.24516129032259No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG5647.27741935483871No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC610.7870967741935483No Hit
TGGCTCATCTTCAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT560.7225806451612903No Hit
TGGCTATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT520.6709677419354839No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG470.6064516129032258No Hit
TGGCTCATCTTAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT400.5161290322580645No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC390.5032258064516129No Hit
TGGCTCTCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT370.4774193548387097No Hit
TGGCTCATTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT300.3870967741935484No Hit
TGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT260.33548387096774196No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG260.33548387096774196No Hit
TGGCTCATCTTCAAACCGTGGGCAACTCTGGCCGGCCTCTGCTGAATGAC220.2838709677419355No Hit
GGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT220.2838709677419355No Hit
TGGTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT190.24516129032258063No Hit
TGGCTCATCTTCAAACCGTCCCCTGTTTTGTAGTCCAACCCTGTGATGAT180.23225806451612901No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGGTGAT180.23225806451612901No Hit
TGGCTCATCTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT150.1935483870967742No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTGGTCCAACCCTGTGATGAT150.1935483870967742No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCAGTGATGAT140.18064516129032257No Hit
TGGCTCATCTTCAAACGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT120.15483870967741936No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCCGTGATGAT110.14193548387096774No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGTTGAGGTGTAGAACCATGTCGT110.14193548387096774No Hit
TGGCTCATCTTCAAACCGTTTCCTGTTTTGTAGTCCAACCCTGTGATGAT110.14193548387096774No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGCGATGAT100.12903225806451613No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGCAGTCCAACCCTGTGATGAT90.11612903225806451No Hit
TGGCTCATCTTCAAACCGCCTCCTGTTTTGTAGTCCAACCCTGTGATGAT90.11612903225806451No Hit
TGGCTCATCTTCAAACCGTCTCCCGTTTTGTAGTCCAACCCTGTGATGAT90.11612903225806451No Hit
TGGCCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT90.11612903225806451No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGGT90.11612903225806451No Hit
TGGCTCACTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT90.11612903225806451No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGAAGAT80.1032258064516129No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGCCCAACCCTGTGATGAT80.1032258064516129No Hit
TGACTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT80.1032258064516129No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGAGATGAT80.1032258064516129No Hit
TGGCTCATCTTCAAACCATCTCCTGTTTTGTAGTCCAACCCTGTGATGAT80.1032258064516129No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)