FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008140865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008140865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16107
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC663941.218103929968336No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC274117.017445831005155No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT247015.334947538337367No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAC8935.5441733407835105No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG8305.153039051344136No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCAAACCCTGTGATGAT3011.8687527162103434No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAAACCTGTGATGAT1831.1361519836096108No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT1000.6208480784751972No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG460.28559011609859064No Hit
AGACCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT390.24213075060532688No Hit
TGGCTATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT250.1552120196187993No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG240.1490035388340473No Hit
TGGCTCATCTTCAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT230.14279505804929532No Hit
AGACCCCTGGAACTGCTACATGTGCGGACACAAGGGTACCTACGGGCTGC210.1303780964797914No Hit
AGACCCCTGGAACTGTTACATGTGCGGGCACAAGGGTACCTACGGGCTGC210.1303780964797914No Hit
TGGCTCATCTTAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT200.12416961569503944No Hit
GGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT190.11796113491028745No Hit
AGACCCCTGGAACTGCTACGTGTGCGGGCACAAGGGTACCTACGGGCTGC180.11175265412553548No Hit
TGGCTCTCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT180.11175265412553548No Hit
GACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT180.11175265412553548No Hit
AGACCCCTGGAATGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT180.11175265412553548No Hit
TGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT170.10554417334078352No Hit
AGACCCCTGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT170.10554417334078352No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)