FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008140900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008140900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9200
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA569161.858695652173914No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC109411.891304347826088No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT5135.576086956521739No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT4334.706521739130435No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC2462.6739130434782608No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGAACTGGAGACCAAGTCTCTGC951.0326086956521738No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC580.6304347826086957No Hit
CGTTAGTGAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT500.5434782608695652No Hit
CGTTAGTGACAAGAGGGAATCTCGCGATTTCTCGAGTCCAACCCTGTGAT360.391304347826087No Hit
TGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA250.2717391304347826No Hit
CGTTAGTGACAAGAGGGACATTTCGCGATTTCTCGAGTCCAACCCTGTGA210.2282608695652174No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT200.21739130434782608No Hit
CGTTAGTGACAAGAGGGACATCTTGCGATTTCTCGAGTCCAACCCTGTGA190.20652173913043476No Hit
CGTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT170.18478260869565216No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC160.17391304347826086No Hit
CGTTAGTGACAAGAGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT150.16304347826086957No Hit
CGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT140.15217391304347827No Hit
CGTTAGAGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA140.15217391304347827No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCCGTGA130.14130434782608697No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCCCGAGTCCAACCCTGTGA130.14130434782608697No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGG120.13043478260869568No Hit
CGTTAGTGACAAGAGGGACTTCTCGCGATTTCTCGAGTCCAACCCTGTGA120.13043478260869568No Hit
CTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT110.11956521739130434No Hit
CGTTAGTGGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTG110.11956521739130434No Hit
CGTTAGTGACAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT100.10869565217391304No Hit
CGTTAGTGACAAGAGGGACATCTCGCAATTTCTCGAGTCCAACCCTGTGA100.10869565217391304No Hit
CGTTGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT100.10869565217391304No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGCCCAACCCTGTGA100.10869565217391304No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)