FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008140901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008140901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9200
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCAC561461.02173913043478No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG107911.728260869565217No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT4584.978260869565218No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT4134.489130434782608No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC1511.6413043478260871No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC1101.1956521739130435No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG981.065217391304348No Hit
GCTATCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA640.6956521739130435No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTAGAGGGTCAAATT590.6413043478260869No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG570.6195652173913043No Hit
GCTATCCTGCCATGCTCCGGACACTCCTGCAGCTCCAGCTTATCATTCAC520.5652173913043479No Hit
GTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA380.4130434782608695No Hit
GCTATCCTGCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA310.33695652173913043No Hit
GCTATCCGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA220.23913043478260868No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGTTGAGGTGTAGAACCATGTCGT210.2282608695652174No Hit
GCTACCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA200.21739130434782608No Hit
CTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA190.20652173913043476No Hit
GCTTCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA190.20652173913043476No Hit
GCATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA170.18478260869565216No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCGTTGAGGTGTAGAACCATGTCGT140.15217391304347827No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATCCAC130.14130434782608697No Hit
GCTATCCTGCCATGCTCCAGGCACTCCTGCAGCTCCAGCTTATCATTCAC120.13043478260869568No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCGGCTTATCATTCAC120.13043478260869568No Hit
GCTATCCTGCCTGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA120.13043478260869568No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTACCATTCAC110.11956521739130434No Hit
GCTATCCTGCCATGCTCCAGACACTCCCGCAGCTCCAGCTTATCATTCAC110.11956521739130434No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGTTCCAGCTTATCATTCAC110.11956521739130434No Hit
GCTATCCTCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA100.10869565217391304No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCGGCTCCAGCTTATCATTCAC100.10869565217391304No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGTAGCTCCAGCTTATCATTCAC100.10869565217391304No Hit
GCTATCCTGCCATGCTCCAGACACCCCTGCAGCTCCAGCTTATCATTCAC100.10869565217391304No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)