FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008140930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008140930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14231
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA568639.955027756306656No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT564239.6458435809149No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC12458.74850678097112No Hit
GCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT930.653502916168927No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGAACTGGAGACCAAGTCTCTGC860.6043145246293303No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC600.4216147846251142No Hit
CGTTAGTGAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT450.31621108846883567No Hit
CGTTAGTGACAAGAGGGAATCTCGCGATTTCTCGAGTCCAACCCTGTGAT320.22486121846672755No Hit
CGTTAGTGACAAGAGGGACATCTTGCGATTTCTCGAGTCCAACCCTGTGA280.1967535661583866No Hit
CGTTAGTGACAAGAGGGACATTTCGCGATTTCTCGAGTCCAACCCTGTGA280.1967535661583866No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT250.17567282692713093No Hit
AGCCAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT240.16864591385004568No Hit
CGTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT200.14053826154170473No Hit
AGCAAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT190.13351134846461948No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGGGTACTTTTAAAGGTCCTGCT190.13351134846461948No Hit
AGACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT190.13351134846461948No Hit
AGCACAGGACTCTTCTTTGATATAAAGAGAGTACTTTTAAAGGTCCTGCT180.12648443538753426No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGCCCTGCT170.11945752231044902No Hit
AGCACGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT160.11243060923336377No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACCTTTAAAGGTCCTGCT150.10540369615627855No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)