FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008140931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008140931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14231
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCAC557639.18206731782728No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT529737.22155856932049No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG12378.692291476354438No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG850.5972876115522451No Hit
TGGCTATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT670.4708031761647109No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG590.4145878715480289No Hit
GCTATCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA470.3302649146230061No Hit
GCTATCCTGCCATGCTCCGGACACTCCTGCAGCTCCAGCTTATCATTCAC440.3091841753917504No Hit
TGGCTCATCTTCAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT430.30215726231466516No Hit
TGGCTCATCTTAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT390.2740496100063242No Hit
GGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT360.2529688707750685No Hit
GCTATCCTGCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA350.24594195769798327No Hit
TGGCTCATCTTCAAACCGTGGGCAACTCTGGCCGGCCTCTGCTGAATGAC330.2318881315438128No Hit
GCATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA310.21783430538964235No Hit
TGGCTCTCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT310.21783430538964235No Hit
TGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT270.18972665308130138No Hit
GTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA260.18269974000421615No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGTTGAGGTGTAGAACCATGTCGT230.16161900077296046No Hit
GCTACCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA220.1545920876958752No Hit
TGGCTCATTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT220.1545920876958752No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCAGTGATGAT190.13351134846461948No Hit
GCTTCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA190.13351134846461948No Hit
GCTATCCTGCCATGCTCCAGACACTCCCGCAGCTCCAGCTTATCATTCAC190.13351134846461948No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGGTGAT180.12648443538753426No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCCTATCATTCAC180.12648443538753426No Hit
GCTATCCGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA180.12648443538753426No Hit
GCTATCCTGCCATGCTCCAGACACCCCTGCAGCTCCAGCTTATCATTCAC160.11243060923336377No Hit
TGGTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT160.11243060923336377No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGCGATGAT150.10540369615627855No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)