FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008140938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008140938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13265
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA926469.83791933660008No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC11318.526196758386732No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC6925.216735770825481No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT2231.6811157180550322No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT2091.5755748209574065No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC1200.9046362608367886No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGAACTGGAGACCAAGTCTCTGC1120.8443271767810026No Hit
CGTTAGTGAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT760.5729362985299661No Hit
CGTTAGTGACAAGAGGGAATCTCGCGATTTCTCGAGTCCAACCCTGTGAT610.45985676592536745No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC570.42970222389747453No Hit
CGTTAGTGACAAGAGGGACATTTCGCGATTTCTCGAGTCCAACCCTGTGA440.33169996230682247No Hit
CGTTAGTGACAAGAGGGACATCTTGCGATTTCTCGAGTCCAACCCTGTGA390.2940067847719563No Hit
TGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA280.21108179419525064No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGCCCAACCCTGTGA280.21108179419525064No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGG240.18092725216735772No Hit
CGTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT240.18092725216735772No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCGACCCTGTGA200.15077271013946475No Hit
CGTTATGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT190.14323407463249152No Hit
CGTTAGTGACAAGAGGGACTCTCGCGATTTCTCGAGTCCAACCCTGTGAT190.14323407463249152No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCCGTGA190.14323407463249152No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT190.14323407463249152No Hit
CGTTAGTGACAAGAGGGACTTCTCGCGATTTCTCGAGTCCAACCCTGTGA170.12815680361854503No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCAGTGA150.11307953260459858No Hit
CGTTAGTGACAAGAGGGACATCTCGCGACTTCTCGAGTCCAACCCTGTGA150.11307953260459858No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAGCCCTGTGA150.11307953260459858No Hit
CTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT150.11307953260459858No Hit
CGTTAGAGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA150.11307953260459858No Hit
CGTTAGTGCAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT150.11307953260459858No Hit
CGGAAACACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT140.10554089709762532No Hit
CGTTGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT140.10554089709762532No Hit
CGTTAGTGACAAGAGGGACATCTCACGATTTCTCGAGTCCAACCCTGTGA140.10554089709762532No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)