FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008140968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008140968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8499
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT615172.3732203788681No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC5336.271326038357454No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC5276.200729497587951No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT3013.541593128603365No Hit
GCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT1061.2472055535945406No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGAACTGGAGACCAAGTCTCTGC450.5294740557712672No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC380.4471114248735145No Hit
AGACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT280.3294505235910107No Hit
AGCAAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT210.24708789269325804No Hit
AGCCAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT170.2000235321802565No Hit
ACACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT170.2000235321802565No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGGGTACTTTTAAAGGTCCTGCT150.17649135192375573No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACCTTTAAAGGTCCTGCT150.17649135192375573No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCC140.16472526179550534No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGCCCTGCT130.15295917166725498No Hit
AGCACAGGACTCTTCTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT130.15295917166725498No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCCGCT110.1294269914107542No Hit
AGCACAGGACTCTTCTTTGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT100.11766090128250382No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAGGGTCCTGCT100.11766090128250382No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC100.11766090128250382No Hit
AGCACAGGACTCTTTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT100.11766090128250382No Hit
AGCACAGGACTCTTCTTTGGTATAAGGAGAGTACTTTTAAAGGTCCTGCT90.10589481115425345No Hit
AGCACAGGATCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT90.10589481115425345No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)