FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008140980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008140980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23967
Total Bases3.4 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA729330.429340342971585No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC725830.283306212709142No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT421817.59919889848542No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC10434.351817081820837No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT9704.047231610130597No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGAACTGGAGACCAAGTCTCTGC1150.4798264280051738No Hit
CAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT840.35048191262986605No Hit
GCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT650.27120624191596776No Hit
CGTTAGTGAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT640.26703383819418364No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC640.26703383819418364No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC550.22948220469812658No Hit
ACAACAGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT500.20862018608920602No Hit
CGTTAGTGACAAGAGGGAATCTCGCGATTTCTCGAGTCCAACCCTGTGAT470.19610297492385362No Hit
ACACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT460.1919305712020695No Hit
CGTTAGTGACAAGAGGGACATCTTGCGATTTCTCGAGTCCAACCCTGTGA420.17524095631493303No Hit
ACAACAGGTCAGATGCAGGTCCAGCGGTGTCCCAAGCTCCCACCCAGCGC410.1710685525931489No Hit
ACAACGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT370.15437893770601244No Hit
ACAACAGGTAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT370.15437893770601244No Hit
ACAAAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT330.13768932281887594No Hit
TGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA320.13351691909709182No Hit
CGTTAGTGACAAGAGGGACATTTCGCGATTTCTCGAGTCCAACCCTGTGA300.1251721116535236No Hit
ACAACAGGCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT300.1251721116535236No Hit
ACAACAGGTCGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT280.11682730420995535No Hit
AAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT270.11265490048817123No Hit
ACAACAGGTCAGATGCAGGCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT270.11265490048817123No Hit
ACAACAGGTCAGTGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT260.10848249676638712No Hit
CGTTAGTGACAAGAGGGACTCTCGCGATTTCTCGAGTCCAACCCTGTGAT250.10431009304460301No Hit
ACAACAGGTCAATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT250.10431009304460301No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)